IL-6 and IL-10 Levels in Rats Blood Plasma as Immune Responses Post Radioiodine (I-131) Administration

Harry Nugroho Eko Surniyantoro, Abinawanto, Anom Bowolaksono, Mukh Syaifudin, Fadhillah Fadhillah, Retno Lestari, Teja Kisnanto, Dwi Ramadhani, Indra Saptiama, Iin Kurnia Hasan Basri, Triningsih Triningsih, Bisma Barron Patrianesha

Research output: Contribution to journalArticlepeer-review


OBJECTIVE: The purpose of this study was to analyze the effect of oral administration of radioiodine (I-131) on the immune responses (interleukin 6 and 10) as biodosimetry markers and to support clinical trials of I-131 solution. METHODS: The design of this study was an in vivo experimental study using twenty-seven male rats (Rattus norvegicus strain Sprague-Dawley) given 100 μL of I-131 solution at a dose of 260 μCi. Blood plasma was taken at 0.25, 0.5, 1, 3, 24, 48, 120, and 168 hours post oral I-131 administration, respectively. Rats without radioiodine administration as a control group. The levels of IL-6 and IL-10 were measured using the enzyme-linked immunosorbent assay (ELISA) method. Statistical analysis was carried out with one-way ANOVA using SPSS version 25 software. RESULT: IL-6 level began to significantly increase at 0.25 hours post administration of I-131 (14.4 pg/mL ± 2.52 pg/mL, p=0.02). During 7 days of observation, IL-6 levels had 2 peaks of highly significant increase at 0.5 hours (43.57 ± 5.28, p<0.001) and 120 hours (24.08 ± 2.69, p<0.001 compared to control (5.44 ± 0.95 pg/mL). IL-10 level began to significantly increase at 0.25 hours (30.32 ± 3.22 pg/mL, p=0.03) compared to controls (20.61 ± 1.59 pg/mL). CONCLUSION: The highest increase in IL-6 and IL-10 levels occurred respectively in the first 0.5 hours 8 times and in the first 0.25 hours 1.47 times compared to controls. Internal irradiation with radioiodine resulted in a significant increase in immune cells in exposed blood plasma characterized by the production of the cytokines IL-6 and IL-10. This appears to be a response of immune cells to reduce or stop inflammatory reactions through the release of anti-inflammatory cytokines in an effort to prevent excessive inflammatory responses that can damage cells and tissues.

Original languageEnglish
Pages (from-to)1017-1023
Number of pages7
JournalAsian Pacific journal of cancer prevention : APJCP
Issue number3
Publication statusPublished - 1 Mar 2024


  • Immune responses
  • interleukin 10
  • Interleukin 6
  • radioiodine
  • Rats blood plasma


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