IgG Subclasses Identification of Immunized Mice Sera with Dengue Tetravalent DNA Vaccine Based on prM-E Genes

Dengue fever is still a serious health problem in the world. DENV consists of 11 kb of single positive-stranded RNAencoding three structural proteins and seven non-structural proteins. PrM and E proteins are the main targets of the antibody response that rich of epitopes and able to induce protective immunity. There are four DENV serotypes that have similar antigenic structures in the amino acid sequence of protein E. In our previous study, we successfully constructed a recombinant tetravalent DNA vaccine candidate consisting pUMVC4a-based expression plasmid for prM-E protein of all DENV serotypes (pUMD1, pUMD2, pUMD3 and pUMD4). It has been proved that the vaccine candidate was able to induced anti-dengue IgG as well as neutralization antibody to all DENV serotypes. This study aims to determine IgG subclasses of immunized mice with recombinant tetravalent DNAvaccine candidates based on prM-E genes of all serotypes.