TY - JOUR
T1 - Identification of sperm-bearing female-specific chromosome in the sex-reversed chicken
AU - Abinawanto, null
AU - Zhang, C.
AU - Saito, N.
AU - Matsuda, Y.
AU - Shimada, K.
PY - 1998/1/1
Y1 - 1998/1/1
N2 - Sexual differentiation in the female chick embryo was phenotypically reversed to the male sex by injection of an aromatase inhibitor (Fadrozole, 0.1 mg/egg) into the embryo at day 5 of incubation. The birds were raised to 10 months of age, and the morphology of the gonads of sex-reversed hens were examined by the light-microscopic morphology, and the presence of the W chromosome gene was determined by fluorescence in situ hybridization (FISH) technique and used for PCR analysis of a single isolated sperm. The sex-reversed hens possessed two testes with a fully developed oviduct on the left side. The testes contained essentially the same cellular components as those of normal testes, although sperm counts were low. FISH analysis revealed numerous spermatids and several sperm bearing W-chromosomes, indicating that the second meiosis occurred normally but that the transformation from the spermatid to the spermatozoon is partially impaired. PCR analysis using the DNA of a single sperm also indicates that sperm-carrying the W chromosome were produced.
AB - Sexual differentiation in the female chick embryo was phenotypically reversed to the male sex by injection of an aromatase inhibitor (Fadrozole, 0.1 mg/egg) into the embryo at day 5 of incubation. The birds were raised to 10 months of age, and the morphology of the gonads of sex-reversed hens were examined by the light-microscopic morphology, and the presence of the W chromosome gene was determined by fluorescence in situ hybridization (FISH) technique and used for PCR analysis of a single isolated sperm. The sex-reversed hens possessed two testes with a fully developed oviduct on the left side. The testes contained essentially the same cellular components as those of normal testes, although sperm counts were low. FISH analysis revealed numerous spermatids and several sperm bearing W-chromosomes, indicating that the second meiosis occurred normally but that the transformation from the spermatid to the spermatozoon is partially impaired. PCR analysis using the DNA of a single sperm also indicates that sperm-carrying the W chromosome were produced.
UR - http://www.scopus.com/inward/record.url?scp=0031962709&partnerID=8YFLogxK
U2 - 10.1002/(SICI)1097-010X(19980101)280:1<65::AID-JEZ8>3.0.CO;2-F
DO - 10.1002/(SICI)1097-010X(19980101)280:1<65::AID-JEZ8>3.0.CO;2-F
M3 - Article
C2 - 9437853
AN - SCOPUS:0031962709
SN - 0022-104X
VL - 280
SP - 65
EP - 72
JO - Journal of Experimental Zoology
JF - Journal of Experimental Zoology
IS - 1
ER -