Identification of Brugia malayi in vectors with a species-specific DNA probe

K. L. Sim, J. W. Mak, W. H. Cheong, Inge Sutanto, L. Kurniawan, H. A. Marwoto, E. Franke, J. R. Campell, D. F. Wirth, W. F. Piessens

Research output: Contribution to journalArticlepeer-review

20 Citations (Scopus)

Abstract

We evaluated the potential value of a cloned sequence of genomic DNA of Brugia malayi as a species-specific probe. Clone pBm 15 reacted with all stages of 8 different geographic isolates of B. malayi and cross-hybridized with microfilariae of B. timori. It did not hybridize with Wuchereria bancrofti or with B. pahangi, W. kalimantani, Dirofilaria repens, Breinlia booliati or Cardiofilaria species, animal filariids that can be sympatric with B. malayi. P32-labeled clone pBm 15 correctly identified mosquitoes infected even with 1 infective larva of B. malayi. This specific DNA probe should be an invaluable tool to monitor control programs of Brugian filariasis.

Original languageEnglish
Pages (from-to)559-564
Number of pages6
JournalAmerican Journal of Tropical Medicine and Hygiene
Volume35
Issue number3
Publication statusPublished - 27 Aug 1986

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