Identification of Brugia malayi in vectors with a species-specific DNA probe

K. L. Sim; J. W. Mak; W. H. Cheong; Inge Sutanto; L. Kurniawan; H. A. Marwoto; E. Franke; J. R. Campell; D. F. Wirth; W. F. Piessens

doi:10.4269/ajtmh.1986.35.559

Identification of Brugia malayi in vectors with a species-specific DNA probe

K. L. Sim, J. W. Mak, W. H. Cheong, Inge Sutanto, L. Kurniawan, H. A. Marwoto, E. Franke, J. R. Campell, D. F. Wirth, W. F. Piessens

Department of Parasitology Pre Clinic

Research output: Contribution to journal › Article › peer-review

21 Citations (Scopus)

Abstract

We evaluated the potential value of a cloned sequence of genomic DNA of Brugia malayi as a species-specific probe. Clone pBm 15 reacted with all stages of 8 different geographic isolates of B. malayi and cross-hybridized with microfilariae of B. timori. It did not hybridize with Wuchereria bancrofti or with B. pahangi, W. kalimantani, Dirofilaria repens, Breinlia booliati or Cardiofilaria species, animal filariids that can be sympatric with B. malayi. P³²-labeled clone pBm 15 correctly identified mosquitoes infected even with 1 infective larva of B. malayi. This specific DNA probe should be an invaluable tool to monitor control programs of Brugian filariasis.

Original language	English
Pages (from-to)	559-564
Number of pages	6
Journal	American Journal of Tropical Medicine and Hygiene
Volume	35
Issue number	3
DOIs	https://doi.org/10.4269/ajtmh.1986.35.559
Publication status	Published - 1986

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title = "Identification of Brugia malayi in vectors with a species-specific DNA probe",

abstract = "We evaluated the potential value of a cloned sequence of genomic DNA of Brugia malayi as a species-specific probe. Clone pBm 15 reacted with all stages of 8 different geographic isolates of B. malayi and cross-hybridized with microfilariae of B. timori. It did not hybridize with Wuchereria bancrofti or with B. pahangi, W. kalimantani, Dirofilaria repens, Breinlia booliati or Cardiofilaria species, animal filariids that can be sympatric with B. malayi. P32-labeled clone pBm 15 correctly identified mosquitoes infected even with 1 infective larva of B. malayi. This specific DNA probe should be an invaluable tool to monitor control programs of Brugian filariasis.",

author = "Sim, {K. L.} and Mak, {J. W.} and Cheong, {W. H.} and Inge Sutanto and L. Kurniawan and Marwoto, {H. A.} and E. Franke and Campell, {J. R.} and Wirth, {D. F.} and Piessens, {W. F.}",

year = "1986",

doi = "10.4269/ajtmh.1986.35.559",

language = "English",

volume = "35",

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T1 - Identification of Brugia malayi in vectors with a species-specific DNA probe

AU - Sim, K. L.

AU - Mak, J. W.

AU - Cheong, W. H.

AU - Sutanto, Inge

AU - Kurniawan, L.

AU - Marwoto, H. A.

AU - Franke, E.

AU - Campell, J. R.

AU - Wirth, D. F.

AU - Piessens, W. F.

PY - 1986

Y1 - 1986

N2 - We evaluated the potential value of a cloned sequence of genomic DNA of Brugia malayi as a species-specific probe. Clone pBm 15 reacted with all stages of 8 different geographic isolates of B. malayi and cross-hybridized with microfilariae of B. timori. It did not hybridize with Wuchereria bancrofti or with B. pahangi, W. kalimantani, Dirofilaria repens, Breinlia booliati or Cardiofilaria species, animal filariids that can be sympatric with B. malayi. P32-labeled clone pBm 15 correctly identified mosquitoes infected even with 1 infective larva of B. malayi. This specific DNA probe should be an invaluable tool to monitor control programs of Brugian filariasis.

AB - We evaluated the potential value of a cloned sequence of genomic DNA of Brugia malayi as a species-specific probe. Clone pBm 15 reacted with all stages of 8 different geographic isolates of B. malayi and cross-hybridized with microfilariae of B. timori. It did not hybridize with Wuchereria bancrofti or with B. pahangi, W. kalimantani, Dirofilaria repens, Breinlia booliati or Cardiofilaria species, animal filariids that can be sympatric with B. malayi. P32-labeled clone pBm 15 correctly identified mosquitoes infected even with 1 infective larva of B. malayi. This specific DNA probe should be an invaluable tool to monitor control programs of Brugian filariasis.

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ER -

Identification of Brugia malayi in vectors with a species-specific DNA probe

Abstract

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