TY - GEN
T1 - Identification of Brucella abortus Biovars Isolated from Cattle in Indonesia
AU - Noor, S. M.
AU - Pratiwi, P. S.
AU - Asmarani, K.
AU - Karuniawati, A.
AU - Sumirah,
N1 - Funding Information:
The authors are grateful for financial support from the DIPA-APBN project supported by Indonesian Government funds. We thank all technical staff at Bacteriology Laboratory, BBLitvet for their support on the laboratory work on identification and bio-typing test.
Publisher Copyright:
© 2023 American Institute of Physics Inc.. All rights reserved.
PY - 2023/6/5
Y1 - 2023/6/5
N2 - Bovine brucellosis is a bacterial zoonotic disease and is endemic in Indonesia causing significant economic losses due to abortion, stillbirth, infertility, sterility, and reduced milk production. Brucella abortus consists of eight biovars (1, 2, 3, 4, 5, 6, 7, and 9) based on geographic distribution. There are limited studies concerning the identification of B. abortus biovars in Indonesia, in fact, for the epidemiology survey, biovars is a critical component for control of bovine brucellosis properly. The aim of this study was to define whether most of B. abortus biovar is accountable for Indonesian bovine brucellosis. Fifty isolates of B. abortus were isolated from milk samples (n=29) and hygroma fluid (n=21) and examined for biovar identification. The identification of biovars using classical biotyping based on the agglutination mono-specific antisera, CO2 requirement for growth, H2S production, and growth in presence of basic fuchsin and thionin dyes. The results showed that 42 isolates (84%) were B. abortus biovar 1, 2 isolates were biovar 2 (12%), and 3 isolates were biovar 3 (4%). The present study revealed that B. abortus biovar 1 has remained to be the most prevalent cause of bovine brucellosis in Indonesia.
AB - Bovine brucellosis is a bacterial zoonotic disease and is endemic in Indonesia causing significant economic losses due to abortion, stillbirth, infertility, sterility, and reduced milk production. Brucella abortus consists of eight biovars (1, 2, 3, 4, 5, 6, 7, and 9) based on geographic distribution. There are limited studies concerning the identification of B. abortus biovars in Indonesia, in fact, for the epidemiology survey, biovars is a critical component for control of bovine brucellosis properly. The aim of this study was to define whether most of B. abortus biovar is accountable for Indonesian bovine brucellosis. Fifty isolates of B. abortus were isolated from milk samples (n=29) and hygroma fluid (n=21) and examined for biovar identification. The identification of biovars using classical biotyping based on the agglutination mono-specific antisera, CO2 requirement for growth, H2S production, and growth in presence of basic fuchsin and thionin dyes. The results showed that 42 isolates (84%) were B. abortus biovar 1, 2 isolates were biovar 2 (12%), and 3 isolates were biovar 3 (4%). The present study revealed that B. abortus biovar 1 has remained to be the most prevalent cause of bovine brucellosis in Indonesia.
UR - http://www.scopus.com/inward/record.url?scp=85163394277&partnerID=8YFLogxK
U2 - 10.1063/5.0143991
DO - 10.1063/5.0143991
M3 - Conference contribution
AN - SCOPUS:85163394277
T3 - AIP Conference Proceedings
BT - Proceedings of the 4th International Conference of Animal Science and Technology, ICAST 2021
A2 - Dirpan, Andi
A2 - Dagong, Muhammad Ihsan Andi
A2 - Prahesti, Kusumandari Indah
PB - American Institute of Physics Inc.
T2 - 4th International Conference of Animal Science and Technology, ICAST 2021
Y2 - 9 November 2021 through 10 November 2021
ER -