TY - JOUR
T1 - Identification of active compounds in ethyl acetate, chloroform, and N-hexane extracts from peels of Citrus aurantifolia from Maribaya, West Java, Indonesia
AU - Asmah, Nur
AU - Suniarti, Dewi
AU - Margono, Anggraini
AU - Mas'ud, Z.
AU - Bachtiar, Endang
N1 - Publisher Copyright:
© 2020 Wolters Kluwer Medknow Publications. All rights reserved.
PY - 2020/7/1
Y1 - 2020/7/1
N2 - Lime peel contains essential oils are used as anti-oxidants or anti-cancer compounds. As a traditional medicine, lime has been widely used as a substitute for antibiotics. This study aimed to identify active compounds in peel extracts from Citrus aurantifolia that grows in Indonesia. Extraction was carried out by maceration using three different solvents: ethyl acetate, chloroform, and n-hexane. The extracts were analyzed using gas chromatography-mass spectrometry. The results showed that lime peel contained many important compounds and that 28, 27, and 24 different chemical compounds, both minor and major constituents, were extracted by ethyl acetate, chloroform, and n-hexane, respectively. Four compounds were found in all three solvent extracts, namely, D-limonene, phytol, α-tocopherol, and 5, 7-dimethoxycoumarin. Forty-seven compounds were uniquely present in one solvent, including 17 in ethyl acetate, 17 in chloroform, and 13 in n-hexane. Among the active compounds extracted, several are of biological importance, for example, stigmasterol, D-limonene, Vitamin E, and α-tocopherol. It can be concluded that a variety of distinct compounds are extracted from the same lime peel sample when different solvents are used and that some of these are bioactive compounds with anti-oxidant, anti-microbial, or anti-cancer properties.
AB - Lime peel contains essential oils are used as anti-oxidants or anti-cancer compounds. As a traditional medicine, lime has been widely used as a substitute for antibiotics. This study aimed to identify active compounds in peel extracts from Citrus aurantifolia that grows in Indonesia. Extraction was carried out by maceration using three different solvents: ethyl acetate, chloroform, and n-hexane. The extracts were analyzed using gas chromatography-mass spectrometry. The results showed that lime peel contained many important compounds and that 28, 27, and 24 different chemical compounds, both minor and major constituents, were extracted by ethyl acetate, chloroform, and n-hexane, respectively. Four compounds were found in all three solvent extracts, namely, D-limonene, phytol, α-tocopherol, and 5, 7-dimethoxycoumarin. Forty-seven compounds were uniquely present in one solvent, including 17 in ethyl acetate, 17 in chloroform, and 13 in n-hexane. Among the active compounds extracted, several are of biological importance, for example, stigmasterol, D-limonene, Vitamin E, and α-tocopherol. It can be concluded that a variety of distinct compounds are extracted from the same lime peel sample when different solvents are used and that some of these are bioactive compounds with anti-oxidant, anti-microbial, or anti-cancer properties.
KW - Anti-microbial
KW - bioactive compounds
KW - Citrus aurantifolia
KW - gas chromatography-ass spectrometry
KW - lime peel
UR - http://www.scopus.com/inward/record.url?scp=85089303656&partnerID=8YFLogxK
U2 - 10.4103/japtr.JAPTR_177_19
DO - 10.4103/japtr.JAPTR_177_19
M3 - Article
AN - SCOPUS:85089303656
VL - 11
SP - 107
EP - 112
JO - Journal of Advanced Pharmaceutical Technology and Research
JF - Journal of Advanced Pharmaceutical Technology and Research
SN - 0110-5558
IS - 3
ER -