TY - JOUR
T1 - Identification and characterization of lignin depolymerization enzymes in Bacillus subtilis strain S11Y isolated from a tropical environment in Malaysia
AU - Riyadi, Fatimah Azizah
AU - Azman, Nadia Farhana
AU - Akhir, Fazrena Nadia Md
AU - Othman, Nor’Azizi
AU - Hara, Hirofumi
N1 - Publisher Copyright:
© 2023 Applied Microbiology, Molecular and Cellular Biosciences Research Foundation.
PY - 2023
Y1 - 2023
N2 - Biological pretreatment using microbial enzymes appears to be the most promising pre-treatment technology for the breakdown of recalcitrant lignin structure. This research focuses on the identification and characterization of lignin-depolymerizing enzymes in Bacillus subtilis strain S11Y, previously isolated from palm oil wastes in Malaysia. The draft genome sequences of this highly lignin-depolymerizing strain revealed that the genome lacked any of the well-known dye-decolorizing per-oxidase or catalase-peroxidase that are commonly reported to be involved in lignin depolymerization by bacteria, indicating that strain S11Y has distinct sets of potential lignin depolymerization genes. The oxidative stress-related enzymes Cu/Zn type-superoxide dismutase (Sod2) and a heme-containing monofunctional catalase (Kat2) were identified in the genome sequences that are of interest. Their lignin-depolymerizing ability were evaluated by treating Alkali lignin (AL) with each enzyme and their degradation ability were evaluated using gel-permeation chromatography (GPC), ultra-high-pressure liquid chromatography–mass spec-trometry (UHPLC/MS), and gas chromatography– mass spectrometry (GC/MS), which successfully proved lignin depolymerizing ability. Successful evaluation of lignin depolymerizing enzymes can be applicable for lignin pre-treatment process in green energy production and generation of valuable chemicals in bio-refinery.
AB - Biological pretreatment using microbial enzymes appears to be the most promising pre-treatment technology for the breakdown of recalcitrant lignin structure. This research focuses on the identification and characterization of lignin-depolymerizing enzymes in Bacillus subtilis strain S11Y, previously isolated from palm oil wastes in Malaysia. The draft genome sequences of this highly lignin-depolymerizing strain revealed that the genome lacked any of the well-known dye-decolorizing per-oxidase or catalase-peroxidase that are commonly reported to be involved in lignin depolymerization by bacteria, indicating that strain S11Y has distinct sets of potential lignin depolymerization genes. The oxidative stress-related enzymes Cu/Zn type-superoxide dismutase (Sod2) and a heme-containing monofunctional catalase (Kat2) were identified in the genome sequences that are of interest. Their lignin-depolymerizing ability were evaluated by treating Alkali lignin (AL) with each enzyme and their degradation ability were evaluated using gel-permeation chromatography (GPC), ultra-high-pressure liquid chromatography–mass spec-trometry (UHPLC/MS), and gas chromatography– mass spectrometry (GC/MS), which successfully proved lignin depolymerizing ability. Successful evaluation of lignin depolymerizing enzymes can be applicable for lignin pre-treatment process in green energy production and generation of valuable chemicals in bio-refinery.
KW - Bacillus subtilis strain S11Y
KW - Cu/Zn type-superoxide dismutase
KW - lignin depolymerization
KW - monofunctional catalase
UR - http://www.scopus.com/inward/record.url?scp=85187155263&partnerID=8YFLogxK
U2 - 10.2323/jgam.2023.08.003
DO - 10.2323/jgam.2023.08.003
M3 - Article
C2 - 37612074
AN - SCOPUS:85187155263
SN - 0022-1260
VL - 69
SP - 278
EP - 286
JO - Journal of General and Applied Microbiology
JF - Journal of General and Applied Microbiology
IS - 5
ER -