TY - JOUR
T1 - Ideal concentration of advanced-platelet rich fibrin (A-PRF) conditioned media for human dental pulp stem cells differentiation
AU - Bagio, Dini Asrianti
AU - Julianto, Indah
AU - Suprastiwi, Endang
AU - Margono, Anggraini
N1 - Funding Information:
Authors’ Contributions: DAB contributed with the project design, data analysis and interpretation, critical content review, and approval of the final version of the manuscript. ES, IJ and AM contributed to the intellectual conceptualizationfoh etusdy,adtanaalysisnaderviewedhteamnuscript. Financial Support: HIBAH Penelitian Dasar Unggulan Perguruan Tinggi (PDUPT) from the Indonesian Ministry of Research and Technology for Higher Education (KEMENRISTEK DIKTI) No. 12703/Ilmu Konservasi Gigi/Genome/2017. Acknowledgements: The authors also thank Emilia Rahmadaniah, S.Si and Yanni Dirgantara, S.Si from PT Prodia Stem Cell Indonesia for the support. Conflict of Interest: The authors declare no conflicts of interest.
Publisher Copyright:
© Pesquisa Brasileira em Odontopediatria e Clinica Integrada 2019. All Rights Reserved.
PY - 2019/1/1
Y1 - 2019/1/1
N2 - To discover the ideal concentration of Advanced Platelet Rich Fibrin (A-PRF) as modification of PRF, for human Dental Pulp Stem Cells (hDPSCs) differentiation. Material and Methods: hDPSCs were devided into five experimental groups: Group I (control group) consist of hDPSCs cultured in 10% FBS, Group II consist of hDPSCs cultured in 1% A-PRF, Group III consist of hDPSCs cultured in 5% A-PRF, Group IV consist of hDPSCs cultured in 10% A-PRF and Group V consist of hDPSCs cultured in 25% A-APRF. All group have been observed for 7 and 14 days and each group had three biological replicates (triplo). Formation of the mineralized nodules was detected after 7 days by Alizarin red-based assay and Dentin Sialophosphoprotein (DSPP) expression after 7 and 14 days quantified by ELISA reader. Statistical analysis was proven with Kruskal-Wallis and post hoc Mann-Whitney test. Results: The differentiation of hDPSCs in all A-PRF groups was significantly different on day-7 (p>0.05) compare to control group (Group I). There were no significant differences between all groups on day-14 (p>0.05). Significantly differences between Group II (1% A-PRF) and Group I (control), Group II (1% APRF) and Group III (5% A-PRF), also Group II (1% A-PRF) and Group V (25% A-PRF) was found from post hoc test analysis. Conclusion: The ideal conditioned media concentration for differentiation of human dental pulp stem cells was on 1% up to 5% A-PRF group.
AB - To discover the ideal concentration of Advanced Platelet Rich Fibrin (A-PRF) as modification of PRF, for human Dental Pulp Stem Cells (hDPSCs) differentiation. Material and Methods: hDPSCs were devided into five experimental groups: Group I (control group) consist of hDPSCs cultured in 10% FBS, Group II consist of hDPSCs cultured in 1% A-PRF, Group III consist of hDPSCs cultured in 5% A-PRF, Group IV consist of hDPSCs cultured in 10% A-PRF and Group V consist of hDPSCs cultured in 25% A-APRF. All group have been observed for 7 and 14 days and each group had three biological replicates (triplo). Formation of the mineralized nodules was detected after 7 days by Alizarin red-based assay and Dentin Sialophosphoprotein (DSPP) expression after 7 and 14 days quantified by ELISA reader. Statistical analysis was proven with Kruskal-Wallis and post hoc Mann-Whitney test. Results: The differentiation of hDPSCs in all A-PRF groups was significantly different on day-7 (p>0.05) compare to control group (Group I). There were no significant differences between all groups on day-14 (p>0.05). Significantly differences between Group II (1% A-PRF) and Group I (control), Group II (1% APRF) and Group III (5% A-PRF), also Group II (1% A-PRF) and Group V (25% A-PRF) was found from post hoc test analysis. Conclusion: The ideal conditioned media concentration for differentiation of human dental pulp stem cells was on 1% up to 5% A-PRF group.
KW - Cell Differentiation
KW - Dental Pulp
KW - Platelet-Rich Fibrin
UR - http://www.scopus.com/inward/record.url?scp=85073787252&partnerID=8YFLogxK
U2 - 10.4034/PBOCI.2019.191.109
DO - 10.4034/PBOCI.2019.191.109
M3 - Article
AN - SCOPUS:85073787252
VL - 19
JO - Pesquisa Brasileira em Odontopediatria e Clinica Integrada
JF - Pesquisa Brasileira em Odontopediatria e Clinica Integrada
SN - 1519-0501
IS - 1
M1 - e4754
ER -