TY - JOUR
T1 - Ideal concentration of advanced-platelet rich fibrin (A-PRF) conditioned media for human dental pulp stem cells differentiation
AU - Bagio, Dini Asrianti
AU - Julianto, Indah
AU - Suprastiwi, Endang
AU - Margono, Anggraini
PY - 2019/1/1
Y1 - 2019/1/1
N2 - To discover the ideal concentration of Advanced Platelet Rich Fibrin (A-PRF) as modification of PRF, for human Dental Pulp Stem Cells (hDPSCs) differentiation. Material and Methods: hDPSCs were devided into five experimental groups: Group I (control group) consist of hDPSCs cultured in 10% FBS, Group II consist of hDPSCs cultured in 1% A-PRF, Group III consist of hDPSCs cultured in 5% A-PRF, Group IV consist of hDPSCs cultured in 10% A-PRF and Group V consist of hDPSCs cultured in 25% A-APRF. All group have been observed for 7 and 14 days and each group had three biological replicates (triplo). Formation of the mineralized nodules was detected after 7 days by Alizarin red-based assay and Dentin Sialophosphoprotein (DSPP) expression after 7 and 14 days quantified by ELISA reader. Statistical analysis was proven with Kruskal-Wallis and post hoc Mann-Whitney test. Results: The differentiation of hDPSCs in all A-PRF groups was significantly different on day-7 (p>0.05) compare to control group (Group I). There were no significant differences between all groups on day-14 (p>0.05). Significantly differences between Group II (1% A-PRF) and Group I (control), Group II (1% APRF) and Group III (5% A-PRF), also Group II (1% A-PRF) and Group V (25% A-PRF) was found from post hoc test analysis. Conclusion: The ideal conditioned media concentration for differentiation of human dental pulp stem cells was on 1% up to 5% A-PRF group.
AB - To discover the ideal concentration of Advanced Platelet Rich Fibrin (A-PRF) as modification of PRF, for human Dental Pulp Stem Cells (hDPSCs) differentiation. Material and Methods: hDPSCs were devided into five experimental groups: Group I (control group) consist of hDPSCs cultured in 10% FBS, Group II consist of hDPSCs cultured in 1% A-PRF, Group III consist of hDPSCs cultured in 5% A-PRF, Group IV consist of hDPSCs cultured in 10% A-PRF and Group V consist of hDPSCs cultured in 25% A-APRF. All group have been observed for 7 and 14 days and each group had three biological replicates (triplo). Formation of the mineralized nodules was detected after 7 days by Alizarin red-based assay and Dentin Sialophosphoprotein (DSPP) expression after 7 and 14 days quantified by ELISA reader. Statistical analysis was proven with Kruskal-Wallis and post hoc Mann-Whitney test. Results: The differentiation of hDPSCs in all A-PRF groups was significantly different on day-7 (p>0.05) compare to control group (Group I). There were no significant differences between all groups on day-14 (p>0.05). Significantly differences between Group II (1% A-PRF) and Group I (control), Group II (1% APRF) and Group III (5% A-PRF), also Group II (1% A-PRF) and Group V (25% A-PRF) was found from post hoc test analysis. Conclusion: The ideal conditioned media concentration for differentiation of human dental pulp stem cells was on 1% up to 5% A-PRF group.
KW - Cell Differentiation
KW - Dental Pulp
KW - Platelet-Rich Fibrin
UR - http://www.scopus.com/inward/record.url?scp=85073787252&partnerID=8YFLogxK
U2 - 10.4034/PBOCI.2019.191.109
DO - 10.4034/PBOCI.2019.191.109
M3 - Article
AN - SCOPUS:85073787252
VL - 19
JO - Pesquisa Brasileira em Odontopediatria e Clinica Integrada
JF - Pesquisa Brasileira em Odontopediatria e Clinica Integrada
SN - 1519-0501
IS - 1
M1 - e4754
ER -