TY - JOUR
T1 - Hypoxic and normoxic-human Wharton’s Jelly mesenchymal stem cell-free lysate for anticancer therapies
AU - Widowati, Wahyu
AU - Jasaputra, Diana Krisanti
AU - Kusuma, Hanna Sari Widya
AU - Rizal, Rizal
AU - Laksmitawati, Dian Ratih
AU - Artie, Dwi Surya
AU - Sholihah, Ika Adhani
AU - Yusepany, Dewani Tediana
AU - Lestari, Riyani
AU - Handayani, Anisa Siwianti
AU - Arumwardana, Seila
AU - Murti, Harry
AU - Bachtiar, Indra
AU - Subangkit, Mawar
N1 - Funding Information:
The authors gratefully acknowledge the financial support from Insinas Riset Individu Pratama (2013 -2015), the Ministry of Research, Technology and Higher Education of the Republic of Indonesia. Our research was supported by the Stem Cell and Cancer Institute, Jakarta, Indonesia, and Biomolecular and Biomedical Research Center, Aretha Medika Utama, Bandung, Indonesia both research methodology and facilities.
Publisher Copyright:
© 2021, Walailak University. All rights reserved.
PY - 2021/5/1
Y1 - 2021/5/1
N2 - Cancer can be defined as a disease in which a group of uncontrolled cells growth by ignoring the rules of normal cell division. Mesenchymal Stem Cells (MSCs) are one of the promising cell-based therapies for anticancer therapies. Preceding research had disclosed that conditioned medium from hypoxic-treated human Wharton’s jelly mesenchymal stem cells (hWJMSCs-hypoCM) and normoxictreated hWJMSCs (hWJMSCs-norCM) can inhibit the proliferation of various cancer cell lines and have no cytotoxic effect on normal cells. The present study, hence, measured the effect of a human Wharton’s jelly stem cells cell-free lysate (hWJMSCs-CL), cultured with hypoxic condition (hWJMSCs-hypoCL), and normoxic condition (hWJMSCs-norCL) towards several cancer cell lines including ovarian (SKOV3), cervical (HeLa), tongue squamosa (HSC3), and liver (HepG2) proliferation. Human mesenchymal stem cells (hMSCs), human fibroblast cells, and mouse fibroblast cells (NIH3T3) were used as control. Both hWJMSCs-CL grown in hypoxic and normoxic conditions could inhibit the proliferation of various cancer cell lines with median inhibitory concentration (IC50) 21.09-95.92 μg/mL and could cause low inhibition of the normal cells with IC50 409.19-799.74 μg/mL. The hWJMSCshypoCL and hWJMSC-norCL could inhibit various cancer and were not harmful to normal cells.
AB - Cancer can be defined as a disease in which a group of uncontrolled cells growth by ignoring the rules of normal cell division. Mesenchymal Stem Cells (MSCs) are one of the promising cell-based therapies for anticancer therapies. Preceding research had disclosed that conditioned medium from hypoxic-treated human Wharton’s jelly mesenchymal stem cells (hWJMSCs-hypoCM) and normoxictreated hWJMSCs (hWJMSCs-norCM) can inhibit the proliferation of various cancer cell lines and have no cytotoxic effect on normal cells. The present study, hence, measured the effect of a human Wharton’s jelly stem cells cell-free lysate (hWJMSCs-CL), cultured with hypoxic condition (hWJMSCs-hypoCL), and normoxic condition (hWJMSCs-norCL) towards several cancer cell lines including ovarian (SKOV3), cervical (HeLa), tongue squamosa (HSC3), and liver (HepG2) proliferation. Human mesenchymal stem cells (hMSCs), human fibroblast cells, and mouse fibroblast cells (NIH3T3) were used as control. Both hWJMSCs-CL grown in hypoxic and normoxic conditions could inhibit the proliferation of various cancer cell lines with median inhibitory concentration (IC50) 21.09-95.92 μg/mL and could cause low inhibition of the normal cells with IC50 409.19-799.74 μg/mL. The hWJMSCshypoCL and hWJMSC-norCL could inhibit various cancer and were not harmful to normal cells.
KW - Anticancer
KW - Cell-free lysate
KW - Hypoxia
KW - Mesenchymal stem cells
KW - Wharton’s Jelly
UR - http://www.scopus.com/inward/record.url?scp=85105452571&partnerID=8YFLogxK
U2 - 10.48048/wjst.2021.9270
DO - 10.48048/wjst.2021.9270
M3 - Article
AN - SCOPUS:85105452571
SN - 1686-3933
VL - 18
JO - Walailak Journal of Science and Technology
JF - Walailak Journal of Science and Technology
IS - 9
M1 - 9270
ER -
