TY - JOUR
T1 - Hyaluronic Acid Accelerates VEGF and PDGF Release from Advance Platelet Rich Fibrin in Diabetic Foot Ulcer
AU - Kartika, Ronald Winardi
AU - Alwi, Idrus
AU - Suyatna, Franciscus Dhyanagiri
AU - Sandra, Ferry
AU - Yunir, Em
AU - Waspadji, Sarwono
AU - Immanuel, Suzzana
AU - Silalahi, Todung
AU - Sungkar, Saleha
AU - Rachmat, Jusuf
AU - Bardosono, Saptawati
AU - Reksodiputro, Mirta Hediyati
N1 - Funding Information:
This study was funded by Medical Science Doctoral Programme, Universitas Indonesia.
Publisher Copyright:
© 2021. The Indonesian Biomedical Journal. All Rights Reserved.
PY - 2021
Y1 - 2021
N2 - Background: Hyaluronic acid (HA) is an essential component of extracellular matrix and mediates signaling in wound healing. HA could induce growth factor release from Advanced Platelet Rich Fibrin (A-PRF), including Vascular Endothelial Growth Factor (VEGF) and Platelet-derived Growth Factor (PDGF). However, concentrations of the released-VEGF and PDGF have not been clearly disclosed. Therefore, current study was conducted to measure the release of these growth factors in HA + A-PRF gel of diabetic foot ulcer (DFU) subjects. Methods: Twenty DFU subjects were included in the study and treated with A-PRF or HA+A-PRF. A-PRF was derived from autologous peripheral blood and processed with low-speed centrifugation. HA was added with a ratio of 1:0.6. A-PRF or HA + A-PRF was applied topically on DFU. Upper tips of A-PRF or HA + A-PRF gels were collected on day 0, 3 and 7 for measurements of VEGF and PDGF concentrations with Enzyme-linked Immune-sorbent Assay (ELISA) methods. Results: On day-3, both VEGF and PDGF concentrations of HA + A-PRF group were significantly higher than the VEGF (p=0.000) and PDGF (p=0.019) concentrations of A-PRF group. The VEGF and PDGF concentrations were continuously and significantly increased on day-7 of HA + A-PRF group, compared to the VEGF (p=0.000) and PDGF (p=0.004) concentrations of A-PRF group. Conclusion: Combination HA+A-PRF induces VEGF and PDGF release from A-PRF. A mixture of A-PRF and HA could be more effective than A-PRF alone for treatment of DFU.
AB - Background: Hyaluronic acid (HA) is an essential component of extracellular matrix and mediates signaling in wound healing. HA could induce growth factor release from Advanced Platelet Rich Fibrin (A-PRF), including Vascular Endothelial Growth Factor (VEGF) and Platelet-derived Growth Factor (PDGF). However, concentrations of the released-VEGF and PDGF have not been clearly disclosed. Therefore, current study was conducted to measure the release of these growth factors in HA + A-PRF gel of diabetic foot ulcer (DFU) subjects. Methods: Twenty DFU subjects were included in the study and treated with A-PRF or HA+A-PRF. A-PRF was derived from autologous peripheral blood and processed with low-speed centrifugation. HA was added with a ratio of 1:0.6. A-PRF or HA + A-PRF was applied topically on DFU. Upper tips of A-PRF or HA + A-PRF gels were collected on day 0, 3 and 7 for measurements of VEGF and PDGF concentrations with Enzyme-linked Immune-sorbent Assay (ELISA) methods. Results: On day-3, both VEGF and PDGF concentrations of HA + A-PRF group were significantly higher than the VEGF (p=0.000) and PDGF (p=0.019) concentrations of A-PRF group. The VEGF and PDGF concentrations were continuously and significantly increased on day-7 of HA + A-PRF group, compared to the VEGF (p=0.000) and PDGF (p=0.004) concentrations of A-PRF group. Conclusion: Combination HA+A-PRF induces VEGF and PDGF release from A-PRF. A mixture of A-PRF and HA could be more effective than A-PRF alone for treatment of DFU.
KW - advanced platelet rich fibrin
KW - diabetic foot ulcer
KW - growth factor
KW - hyaluronic acid
KW - PDGF
KW - PRF
KW - VEGF
UR - http://www.scopus.com/inward/record.url?scp=85117336402&partnerID=8YFLogxK
U2 - 10.18585/inabj.v13i3.1523
DO - 10.18585/inabj.v13i3.1523
M3 - Article
AN - SCOPUS:85117336402
SN - 2085-3297
VL - 13
SP - 332
EP - 336
JO - Indonesian Biomedical Journal
JF - Indonesian Biomedical Journal
IS - 3
ER -
