Human Wharton's jelly mesenchymal stem cells inhibit cytokine storm in acute respiratory distress syndrome in a rat model

Wahyu Widowati, Teresa Wargasetia, Fanny Rahardja, Rimonta Gunanegara, Didik Priyandoko, Marisca Gondokesumo, Ervi Afifah, Cahyaning Wijayanti, Rizal Rizal

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

Objective: To evaluate the potential effect of human Wharton's jelly mesenchymal stem cells (hWJMSCs) on acute respiratory distress syndrome in lipopolysaccharide (LPS)-induced rats. Methods: The hWJMSCs (5×10 4 /mL, 5×10 5 /mL, 5×10 6 /mL) were administered to rats on day 1 and day 8 after being induced by LPS (5 mg/kg body weight). TNF-α levels in the lung and IL-18 and IL-1β levels in the serum were measured using ELISA. In addition, caspase-1 expression in lung tissues was quantified using qRT-PCR, and NF-κB and IL-6 expressions were assessed using immunohistochemistry. Results: The hWJMSCs decreased TNF-α levels in the lung and plasma IL-18 and IL-1β levels. Moreover, the hWJMSCs downregulated the expressions of caspase-1, IL-6, and NF-κB in lung tissues. Conclusions: The hWJMSCs can decrease inflammatory markers of acute respiratory distress syndrome in a rat model and may be further investigated for the treatment of acute respiratory distress syndrome.

Original languageEnglish
Pages (from-to)343-350
Number of pages8
JournalAsian Pacific Journal of Tropical Biomedicine
Volume12
Issue number8
DOIs
Publication statusPublished - 1 Aug 2022

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