The aims of this study is to know the association FSH level with spermatogenic histology on testical biopsy and to evaluate the use of FSH level as predictor of the succes of testical biopsy on azoospermic patient using Johnson criteria. This is a cross-sectional study, comparing two groups, i.e. spermatogenic histology pattern using Johnson criteria and FSH level (IU/L). Age, FSH level, spermatogenic histology pattern are collected from medical record. Population of this study were azoospermic patients in the Deparment of Urology Cipto Mangunkusumo hospital and Morula Clinic Bunda Hospital between 2011 – 2015. Descriptive study was calculate, followed by one way ANOVA comparative test with post-hoc test Duncan and ROC curve analysis to determined the AUC and cut-off value. The age of the patients were 21-51 years old and the most frequent were 31-40 years old (67%). The most frequent histology pattern and Johnson criteraia were maturation arrest (41%) and 5 (27.8%) respectively. Mean level of FSH was 15.83 IU/L with minimum level was 2.35 IU/L and maximaum 41.3 IU/L. There was association between FSH level and spermatogensis histology pattern using Johnsosn criteria, however the validity to predict the outcome of sperm in biopsy in azoospermic patients using FSH level was still low.