TY - JOUR
T1 - Honey effect on sperm motility of kancra fish (Tor soro Valenciennes, 1842) after 48 hours freezing
AU - Putri, B. S.D.
AU - Abinawanto,
AU - Arifin, O. Z.
AU - Kristanto, A. H.
N1 - Publisher Copyright:
© Published under licence by IOP Publishing Ltd.
PY - 2020/2/24
Y1 - 2020/2/24
N2 - Kancra (Tor soro) is a freshwater endemic fish found in specific place of Indonesia, with a drastic decrease in its population due to the varying gonad maturity times between males and females. However, the use of cryopreservation is effective for the synchronization of their gametes using cryoprotectants. Therefore, this study aimed to determine the effect of honey concentration (0%, 5%, 10%, 15%, 20%, and 25%) using 10% of methanol in the spermatozoa motility of kancra fish 48 hours after cryopreservation. The study was conducted in the Research and Development Institute for Freshwater Fish Genetic Resources (Balai Besar Perikanan Budidaya Ikan Tawar, BBPBAT), in Cijeruk, Bogor, West Java. The sperm were collected through the stripping method followed by the evaluation of spermatozoa motility 48 hours after cryopreservation. Furthermore, it was equilibrated for 10 minutes at a temperature of 5 °C, frozen at 10 °C for 48 hours, and thawed for 1 minute at 40 °C. The data were analyzed using ANOVA followed by the Tukey test. Additionally, the ANOVA test showed that honey had effects (P<0.05) on the motility percentage after cryopreservation with a 5% optimum concentration using 85.97±1.91%.
AB - Kancra (Tor soro) is a freshwater endemic fish found in specific place of Indonesia, with a drastic decrease in its population due to the varying gonad maturity times between males and females. However, the use of cryopreservation is effective for the synchronization of their gametes using cryoprotectants. Therefore, this study aimed to determine the effect of honey concentration (0%, 5%, 10%, 15%, 20%, and 25%) using 10% of methanol in the spermatozoa motility of kancra fish 48 hours after cryopreservation. The study was conducted in the Research and Development Institute for Freshwater Fish Genetic Resources (Balai Besar Perikanan Budidaya Ikan Tawar, BBPBAT), in Cijeruk, Bogor, West Java. The sperm were collected through the stripping method followed by the evaluation of spermatozoa motility 48 hours after cryopreservation. Furthermore, it was equilibrated for 10 minutes at a temperature of 5 °C, frozen at 10 °C for 48 hours, and thawed for 1 minute at 40 °C. The data were analyzed using ANOVA followed by the Tukey test. Additionally, the ANOVA test showed that honey had effects (P<0.05) on the motility percentage after cryopreservation with a 5% optimum concentration using 85.97±1.91%.
UR - http://www.scopus.com/inward/record.url?scp=85081173803&partnerID=8YFLogxK
U2 - 10.1088/1755-1315/441/1/012062
DO - 10.1088/1755-1315/441/1/012062
M3 - Conference article
AN - SCOPUS:85081173803
SN - 1755-1307
VL - 441
JO - IOP Conference Series: Earth and Environmental Science
JF - IOP Conference Series: Earth and Environmental Science
IS - 1
M1 - 012062
T2 - 2nd International Conference on Fisheries and Marine Science, InCoFiMS 2019
Y2 - 26 September 2019
ER -