TY - JOUR
T1 - High stable Hb/Pt/BDD electrode for determining acrylamide in coffee samples
AU - Wulandari, R.
AU - Saepudin, E.
AU - Ivandini, T. A.
N1 - Publisher Copyright:
© Published under licence by IOP Publishing Ltd.
Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2020
Y1 - 2020
N2 - A novel electrochemical hemoglobin-platinum-modified boron-doped diamond (Hb/Pt/BDD) biosensor was developed for the detection of acrylamide (AA) in coffee samples. The Hb-Pt-BDD electrode was characterized by cyclic voltammetry (CV), scanning electron microscopy with energy dispersive spectroscopy (SEM-EDS), X-ray diffraction (XRD), Raman spectroscopy, and X-ray photoelectrons (XPS). The cyclic voltammetry of Hb-Pt-BDD in 0.2 M sodium acetate buffer (ABS, pH 4.8) containing acrylamide in the concentration range of 0.00213 to 0.00711 ppb, showed linear responses with a detection limit of 0.00155 ppb. The excellent stability of the prepared Pt-modified BDD was proven through the Pt-BDD reusability by removing the Hb adduct without eliminating Pt on the BDD surface. Finally, employing the biosensor was proposed to determine AA in 1 g of coffee showed the AA content of 15.55 ppb. The result was comparable with the reference method using liquid chromatography-tandem mass spectrometry (LC-MS/MS).
AB - A novel electrochemical hemoglobin-platinum-modified boron-doped diamond (Hb/Pt/BDD) biosensor was developed for the detection of acrylamide (AA) in coffee samples. The Hb-Pt-BDD electrode was characterized by cyclic voltammetry (CV), scanning electron microscopy with energy dispersive spectroscopy (SEM-EDS), X-ray diffraction (XRD), Raman spectroscopy, and X-ray photoelectrons (XPS). The cyclic voltammetry of Hb-Pt-BDD in 0.2 M sodium acetate buffer (ABS, pH 4.8) containing acrylamide in the concentration range of 0.00213 to 0.00711 ppb, showed linear responses with a detection limit of 0.00155 ppb. The excellent stability of the prepared Pt-modified BDD was proven through the Pt-BDD reusability by removing the Hb adduct without eliminating Pt on the BDD surface. Finally, employing the biosensor was proposed to determine AA in 1 g of coffee showed the AA content of 15.55 ppb. The result was comparable with the reference method using liquid chromatography-tandem mass spectrometry (LC-MS/MS).
UR - http://www.scopus.com/inward/record.url?scp=85096497336&partnerID=8YFLogxK
U2 - 10.1088/1757-899X/902/1/012007
DO - 10.1088/1757-899X/902/1/012007
M3 - Conference article
AN - SCOPUS:85096497336
SN - 1757-8981
VL - 902
JO - IOP Conference Series: Materials Science and Engineering
JF - IOP Conference Series: Materials Science and Engineering
IS - 1
M1 - 012007
T2 - 4th International Symposium on Current Progress in Functional Materials, ISCPFM 2019
Y2 - 6 November 2019 through 7 November 2019
ER -