High stable Hb/Pt/BDD electrode for determining acrylamide in coffee samples

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A novel electrochemical hemoglobin-platinum-modified boron-doped diamond (Hb/Pt/BDD) biosensor was developed for the detection of acrylamide (AA) in coffee samples. The Hb-Pt-BDD electrode was characterized by cyclic voltammetry (CV), scanning electron microscopy with energy dispersive spectroscopy (SEM-EDS), X-ray diffraction (XRD), Raman spectroscopy, and X-ray photoelectrons (XPS). The cyclic voltammetry of Hb-Pt-BDD in 0.2 M sodium acetate buffer (ABS, pH 4.8) containing acrylamide in the concentration range of 0.00213 to 0.00711 ppb, showed linear responses with a detection limit of 0.00155 ppb. The excellent stability of the prepared Pt-modified BDD was proven through the Pt-BDD reusability by removing the Hb adduct without eliminating Pt on the BDD surface. Finally, employing the biosensor was proposed to determine AA in 1 g of coffee showed the AA content of 15.55 ppb. The result was comparable with the reference method using liquid chromatography-tandem mass spectrometry (LC-MS/MS).

Original languageEnglish
Article number012007
JournalIOP Conference Series: Materials Science and Engineering
Issue number1
Publication statusPublished - 2020
Event4th International Symposium on Current Progress in Functional Materials, ISCPFM 2019 - Bali, Indonesia
Duration: 6 Nov 20197 Nov 2019


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