High performance liquid chromatography for α-mangostin analysis in mangosteen pericarp extract for routine analysis with photodiode array detector

Muchtaridi Muchtaridi, Moses Prasetio, Nyi Mekar Saptarini, Febrina Amelia Saputri

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

The analytical methods for the standardization of α-mangostin are needed to set up a routine of a validated method for quality control of α-mangostin from Garcinia mangostana L. using HPLC methods. This study was aimed to validate the analytical method of High-Performance Liquid Chromatography (HPLC) for α-mangostin analysis with Photodiode Array (PDA). The analysis was conducted by HPLC with octadecyl silanol column, methanol and acetic acid 1% as a mobile phase and PDA detection at 243 nm. The coefficient correlation of analytical method was was 0.9999, the variation was less than 2%, LOD was 0.671 ppm, and LOQ was 2.174 ppm. The level of α-mangostin in mangosteen pericarp extract from Tasikmalaya was 8.56 ± 0.003%. The analytical method was met the validation criteria. The PDA sensitivity was 68.28% higher than UV-Vis detector.

Original languageEnglish
Pages (from-to)973-978
Number of pages6
JournalRasayan Journal of Chemistry
Volume11
Issue number3
DOIs
Publication statusPublished - 1 Jul 2018

Keywords

  • Mangosteen
  • Photodiode array
  • Sensitivity
  • Validation
  • α-Mangostin

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