Abstract
Introduction: Streptozotocin (STZ), a widely used diabetogenic agent, induces diabetes in animal models, yet crucial details like its preparation and dosage are often overlooked. We induced diabetic Achilles tendinopathy in rats using streptozotocin and excess body fat, assessing Transforming Growth Factor-beta (TGF-β) and interleukin-6 (IL-6) blood levels. This study aimed to create diabetic Achilles tendinopathy model in Sprague-Dawley rats using combination of high-fat diet (HFD) and streptozotocin.
Methods: In this study, 8 male Sprague-Dawley rats were divided into control group (containing 2 rats), 26 g/kgBW of STZ dose group, and 30 g/kgBW of STZ dose group (each containing 3 rats). Over 8 weeks, they underwent a high-fat diet and daily 55% fructose administration. Achilles tendon tissue was histopathologically evaluated using the Bonar score after formalin preservation. Blood serum was analyzed via ELISA to reveal TGF-β and IL-6 levels.
Results: A significant difference in mean Bonar Scores can be observed among the three groups (p = 0.004), despite having no significant results in the post-hoc analysis. However, analysis of TGF-β and IL-6 each did not yield significant results with p = 0.402 and p = 0.236, respectively. Combining HFD, which induces insulin resistance, with low-dose STZ injections, which cause early-cell dysfunction, permits the development of an animal model of T2D. Combining HFD with low-dose STZ injections produces an effective T2D animal model with significant Bonar Score outcomes. The Bonar Score revealed substantial tendon damage in the STZ 26 and STZ 30 treatment groups.
Conclusion: Doses of 30-35 mg/kg of STZ are commonly used, with successful T2D induction observed at lower doses of 15-30 mg/kg in various rat models. The tendon evaluation scale revealed significant damage in the STZ 26 mg/kg and STZ 30 mg/kg groups, confirming the efficacy of this T2D model despite the insignificant result in TGF- β and IL-6 analysis.
Methods: In this study, 8 male Sprague-Dawley rats were divided into control group (containing 2 rats), 26 g/kgBW of STZ dose group, and 30 g/kgBW of STZ dose group (each containing 3 rats). Over 8 weeks, they underwent a high-fat diet and daily 55% fructose administration. Achilles tendon tissue was histopathologically evaluated using the Bonar score after formalin preservation. Blood serum was analyzed via ELISA to reveal TGF-β and IL-6 levels.
Results: A significant difference in mean Bonar Scores can be observed among the three groups (p = 0.004), despite having no significant results in the post-hoc analysis. However, analysis of TGF-β and IL-6 each did not yield significant results with p = 0.402 and p = 0.236, respectively. Combining HFD, which induces insulin resistance, with low-dose STZ injections, which cause early-cell dysfunction, permits the development of an animal model of T2D. Combining HFD with low-dose STZ injections produces an effective T2D animal model with significant Bonar Score outcomes. The Bonar Score revealed substantial tendon damage in the STZ 26 and STZ 30 treatment groups.
Conclusion: Doses of 30-35 mg/kg of STZ are commonly used, with successful T2D induction observed at lower doses of 15-30 mg/kg in various rat models. The tendon evaluation scale revealed significant damage in the STZ 26 mg/kg and STZ 30 mg/kg groups, confirming the efficacy of this T2D model despite the insignificant result in TGF- β and IL-6 analysis.
| Original language | English |
|---|---|
| Pages (from-to) | 1447-1451 |
| Journal | Bali Medical Journal |
| Volume | 13 |
| Issue number | 3 |
| Publication status | Published - 3 Jul 2024 |
Keywords
- High-fat diet
- streptozotocin
- animal model
- achilles diabetic tendinopathy
