TY - JOUR
T1 - Hepatitis C vims RNA detection and HCV genotype in patients with chronic non-A, non-B hepatitis in Jakarta
AU - Widjaja, Suwandhi
AU - Li, Sheng
AU - All, Sugianto
AU - Simon, Sumanto
AU - Sulaiman, Ali
AU - Lesmana, Laurentius M. Adrianto
AU - Yapc, Sing Hiem
N1 - Funding Information:
This work was supportedi n part by a grant from A.B.O.S. Ontwikkelings Samenwerking).
PY - 1995/2
Y1 - 1995/2
N2 - Antibody response in HCV infection may be variable and the variability of the serological response could be due to the differences in HCV strains. Since the distribution of hepatitis C virus genotype has been found to be geographically dependent, it is important to determine the distribution of HCV genotype in various countries with high prevalence of chronic non-A, non-B hepatitis. In this study, serum HCV RNA was examined in 53 patients suspected of chronic non-A, non-B hepatitis with an anti-HCV test as determined by currently available assay. HCV viremia was detected in 48 patients (90.6%). These patients had elevated serum ALT level at the time of HCV RNA determination. Using specific genotype probes, all isolates were classified into three different genotypes. Double and triple infections were also noted. HCV genotype Ib is the predominant genotype found in chronic hepatitis C patients in Jakarta.
AB - Antibody response in HCV infection may be variable and the variability of the serological response could be due to the differences in HCV strains. Since the distribution of hepatitis C virus genotype has been found to be geographically dependent, it is important to determine the distribution of HCV genotype in various countries with high prevalence of chronic non-A, non-B hepatitis. In this study, serum HCV RNA was examined in 53 patients suspected of chronic non-A, non-B hepatitis with an anti-HCV test as determined by currently available assay. HCV viremia was detected in 48 patients (90.6%). These patients had elevated serum ALT level at the time of HCV RNA determination. Using specific genotype probes, all isolates were classified into three different genotypes. Double and triple infections were also noted. HCV genotype Ib is the predominant genotype found in chronic hepatitis C patients in Jakarta.
KW - HCV RNA
KW - HCV genotype
KW - Hepatitis C
KW - Polymerase chain reaction
UR - http://www.scopus.com/inward/record.url?scp=0028891083&partnerID=8YFLogxK
U2 - 10.1016/0166-0934(94)00101-L
DO - 10.1016/0166-0934(94)00101-L
M3 - Article
C2 - 7738137
AN - SCOPUS:0028891083
VL - 51
SP - 169
EP - 175
JO - Journal of Virological Methods
JF - Journal of Virological Methods
SN - 0166-0934
IS - 2-3
ER -