Hepatitis B virus subgenotypes and basal core promoter mutations in Indonesia

Andi Utama, Sigit Purwantomo, Marlinang Diarta Siburian, Rama Dhenni, Rino A. Gani, Irsan Hasan, Andri Sanityoso, Upik Anderiani Miskad, Fardah Akil, Irawan Yusuf, Wenny Astuti Achwan, Soewignjo Soemohardjo, Syafruddin A.R. Lelosutan, Ruswhandi Martamala, Benyamin Lukito, Unggul Budihusodo, Laurentius M. Adrianto Lesmana, Ali Sulaiman, Susan Tai

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31 Citations (Scopus)

Abstract

AIM: To identify the distribution of hepatitis B virus (HBV) subgenotype and basal core promoter (BCP) mutations among patients with HBV-associated liver disease in Indonesia. METHODS: Patients with chronic hepatitis (CH, n = 61), liver cirrhosis (LC, n = 62), and hepatocellular carcinoma (HCC, n = 48) were included in this study. HBV subgenotype was identified based on S or preS gene sequence, and mutations in the HBx gene including the overlapping BCP region were examined by direct sequencing. RESULTS: HBV genotype B (subgenotypes B2, B3, B4, B5 and B7) the major genotype in the samples, accounted for 75.4%, 71.0% and 75.0% of CH, LC and HCC patients, respectively, while the genotype C (subgenotypes C1, C2 and C3) was detected in 24.6%, 29.0%, and 25.0% of CH, LC, and HCC patients, respectively. Subgenotypes B3 (84.9%) and C1 (82.2%) were the main subgenotype in HBV genotype B and C, respectively. Serotype adw2 (84.9%) and adrq+ (89.4%) were the most prevalent in HBV genotype B and C, respectively. Double mutation (A1762T/G1764A) in the BCP was significantly higher in LC (59.7%) and HCC (54.2%) than in CH (19.7%), suggesting that this mutation was associated with severity of liver disease. The T1753V was also higher in LC (46.8%), but lower in HCC (22.9%) and CH (18.0%), suggesting that this mutation may be an indicator of cirrhosis. CONCLUSION: HBV genotype B/B3 and C/C1 are the major genotypes in Indonesia. Mutations in BCP, such as A1762T/G1764A and T1753V, might have an association with manifestations of liver disease.

Original languageEnglish
Pages (from-to)4028-4036
Number of pages9
JournalWorld Journal of Gastroenterology
Volume15
Issue number32
DOIs
Publication statusPublished - 7 Dec 2009

Keywords

  • Basal core promoter mutation
  • Hepatitis B virus
  • Indonesia
  • Liver disease
  • Subgenotype

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