TY - JOUR
T1 - Hepatitis B virus subgenotypes and basal core promoter mutations in Indonesia
AU - Utama, Andi
AU - Purwantomo, Sigit
AU - Siburian, Marlinang Diarta
AU - Dhenni, Rama
AU - Gani, Rino A.
AU - Hasan, Irsan
AU - Sanityoso, Andri
AU - Miskad, Upik Anderiani
AU - Akil, Fardah
AU - Yusuf, Irawan
AU - Achwan, Wenny Astuti
AU - Soemohardjo, Soewignjo
AU - Lelosutan, Syafruddin A.R.
AU - Martamala, Ruswhandi
AU - Lukito, Benyamin
AU - Budihusodo, Unggul
AU - Lesmana, Laurentius M. Adrianto
AU - Sulaiman, Ali
AU - Tai, Susan
PY - 2009
Y1 - 2009
N2 - AIM: To identify the distribution of hepatitis B virus (HBV) subgenotype and basal core promoter (BCP) mutations among patients with HBV-associated liver disease in Indonesia. METHODS: Patients with chronic hepatitis (CH, n = 61), liver cirrhosis (LC, n = 62), and hepatocellular carcinoma (HCC, n = 48) were included in this study. HBV subgenotype was identified based on S or preS gene sequence, and mutations in the HBx gene including the overlapping BCP region were examined by direct sequencing. RESULTS: HBV genotype B (subgenotypes B2, B3, B4, B5 and B7) the major genotype in the samples, accounted for 75.4%, 71.0% and 75.0% of CH, LC and HCC patients, respectively, while the genotype C (subgenotypes C1, C2 and C3) was detected in 24.6%, 29.0%, and 25.0% of CH, LC, and HCC patients, respectively. Subgenotypes B3 (84.9%) and C1 (82.2%) were the main subgenotype in HBV genotype B and C, respectively. Serotype adw2 (84.9%) and adrq+ (89.4%) were the most prevalent in HBV genotype B and C, respectively. Double mutation (A1762T/G1764A) in the BCP was significantly higher in LC (59.7%) and HCC (54.2%) than in CH (19.7%), suggesting that this mutation was associated with severity of liver disease. The T1753V was also higher in LC (46.8%), but lower in HCC (22.9%) and CH (18.0%), suggesting that this mutation may be an indicator of cirrhosis. CONCLUSION: HBV genotype B/B3 and C/C1 are the major genotypes in Indonesia. Mutations in BCP, such as A1762T/G1764A and T1753V, might have an association with manifestations of liver disease.
AB - AIM: To identify the distribution of hepatitis B virus (HBV) subgenotype and basal core promoter (BCP) mutations among patients with HBV-associated liver disease in Indonesia. METHODS: Patients with chronic hepatitis (CH, n = 61), liver cirrhosis (LC, n = 62), and hepatocellular carcinoma (HCC, n = 48) were included in this study. HBV subgenotype was identified based on S or preS gene sequence, and mutations in the HBx gene including the overlapping BCP region were examined by direct sequencing. RESULTS: HBV genotype B (subgenotypes B2, B3, B4, B5 and B7) the major genotype in the samples, accounted for 75.4%, 71.0% and 75.0% of CH, LC and HCC patients, respectively, while the genotype C (subgenotypes C1, C2 and C3) was detected in 24.6%, 29.0%, and 25.0% of CH, LC, and HCC patients, respectively. Subgenotypes B3 (84.9%) and C1 (82.2%) were the main subgenotype in HBV genotype B and C, respectively. Serotype adw2 (84.9%) and adrq+ (89.4%) were the most prevalent in HBV genotype B and C, respectively. Double mutation (A1762T/G1764A) in the BCP was significantly higher in LC (59.7%) and HCC (54.2%) than in CH (19.7%), suggesting that this mutation was associated with severity of liver disease. The T1753V was also higher in LC (46.8%), but lower in HCC (22.9%) and CH (18.0%), suggesting that this mutation may be an indicator of cirrhosis. CONCLUSION: HBV genotype B/B3 and C/C1 are the major genotypes in Indonesia. Mutations in BCP, such as A1762T/G1764A and T1753V, might have an association with manifestations of liver disease.
KW - Basal core promoter mutation
KW - Hepatitis B virus
KW - Indonesia
KW - Liver disease
KW - Subgenotype
UR - http://www.scopus.com/inward/record.url?scp=69449103212&partnerID=8YFLogxK
U2 - 10.3748/wjg.15.4028
DO - 10.3748/wjg.15.4028
M3 - Article
C2 - 19705499
AN - SCOPUS:69449103212
SN - 1007-9327
VL - 15
SP - 4028
EP - 4036
JO - World Journal of Gastroenterology
JF - World Journal of Gastroenterology
IS - 32
ER -