TY - JOUR
T1 - Hematological indices and their correlation with glucose control parameters in a prediabetic rat model
AU - Krisnamurti, Desak Gede Budi
AU - Purwaningsih, Erni H.
AU - Tarigan, Tri Juli Edi
AU - Soetikno, Vivian
AU - Louisa, Melva
N1 - Funding Information:
DGBK, VS, and ML: Contributed to the conception and design of the study, data collection, and manuscript writing. EHP and TJET: Supervised the data collection and analysis. ML was responsible for funding acquisition. All authors read and approved the final manuscript.
Publisher Copyright:
Krisnamurti, et al.
PY - 2022/3
Y1 - 2022/3
N2 - Background and Aim: Chronic hyperglycemia in prediabetic individuals would progress to diabetes and lead to several systemic disruptions, including hematological parameters. This study aimed to investigate the correlation between prediabetes and hematological indices in a prediabetic rat model. Materials and Methods: Eighteen male rats were randomly divided into two groups of nine. Prediabetes was induced in nine rats by a 3-week high-fat and high-glucose diet, followed by low-dose streptozotocin (STZ) injection (30 mg/kg body weight). The oral glucose tolerance test was performed, and the fasting blood glucose (FBG) and insulin levels were measured 72 h after STZ administration. The control group of nine rats was given standard diets. At the end of the 3rd week, the animals fasted overnight before blood collection. Blood samples were drawn and used for the analysis of the FBG and fasting insulin levels and glycated albumin to define prediabetes criteria before hematology analysis. Results: We found a significant increase in the FBG and insulin levels in the prediabetic versus the control group. There were decreases in red blood cells, hemoglobin, and hematocrit levels and red cell distribution in prediabetic rats versus the control. At the same time, a significant increase in the platelet count was observed in the prediabetic group. There was a positive correlation between FBG and lymphocytes and neutrophil-lymphocyte ratio in prediabetic rats. On the other hand, we found a negative correlation between white blood cell count and glycated albumin. Conclusion: Correlations were found in several hematological parameters in the prediabetic rat models. The changes in hematological indices in prediabetic rats may be further used as a valuable indicator of glycemic control.
AB - Background and Aim: Chronic hyperglycemia in prediabetic individuals would progress to diabetes and lead to several systemic disruptions, including hematological parameters. This study aimed to investigate the correlation between prediabetes and hematological indices in a prediabetic rat model. Materials and Methods: Eighteen male rats were randomly divided into two groups of nine. Prediabetes was induced in nine rats by a 3-week high-fat and high-glucose diet, followed by low-dose streptozotocin (STZ) injection (30 mg/kg body weight). The oral glucose tolerance test was performed, and the fasting blood glucose (FBG) and insulin levels were measured 72 h after STZ administration. The control group of nine rats was given standard diets. At the end of the 3rd week, the animals fasted overnight before blood collection. Blood samples were drawn and used for the analysis of the FBG and fasting insulin levels and glycated albumin to define prediabetes criteria before hematology analysis. Results: We found a significant increase in the FBG and insulin levels in the prediabetic versus the control group. There were decreases in red blood cells, hemoglobin, and hematocrit levels and red cell distribution in prediabetic rats versus the control. At the same time, a significant increase in the platelet count was observed in the prediabetic group. There was a positive correlation between FBG and lymphocytes and neutrophil-lymphocyte ratio in prediabetic rats. On the other hand, we found a negative correlation between white blood cell count and glycated albumin. Conclusion: Correlations were found in several hematological parameters in the prediabetic rat models. The changes in hematological indices in prediabetic rats may be further used as a valuable indicator of glycemic control.
KW - diabetes mellitus
KW - high-fat and high-glucose diet
KW - hyperglycemia
KW - neutrophil-lymphocyte ratio
KW - streptozotocin
UR - http://www.scopus.com/inward/record.url?scp=85128114422&partnerID=8YFLogxK
U2 - 10.14202/vetworld.2022.672-678
DO - 10.14202/vetworld.2022.672-678
M3 - Article
AN - SCOPUS:85128114422
SN - 0972-8988
VL - 15
SP - 672
EP - 678
JO - Veterinary World
JF - Veterinary World
IS - 3
ER -
