Growth Kinetic of Bacillus subtilis for Pyrene biodegradation

Hanif Yuliani; Meka Saima Perdani; Meilani Manurung; Muhamad Sahlan; Anondho Wijanarko; Heri Hermansyah

doi:10.1016/j.egypro.2018.10.015

Growth Kinetic of Bacillus subtilis for Pyrene biodegradation

Hanif Yuliani, Meka Saima Perdani, Meilani Manurung, Muhamad Sahlan, Anondho Wijanarko, Heri Hermansyah

Department of Chemical Engineering

Research output: Contribution to journal › Conference article › peer-review

4 Citations (Scopus)

Abstract

Biodegradation of pyrene compound was catalyzed by multi-component enzymes, and the initial dioxygenase gene was used as key Bacillus subtilis C19 enzyme for aromatic ring structure degradation. The bacterial cell growth rates were also analyzed; it was fitted using Monod, Mason-Mallis, Haldane, Andrews, and Aiba kinetics models. The un-competitive inhibition mechanism of high substrate concentration proposed by Andrews model could be best fitted to experimental bacterial growth kinetic data. Further, the maximum specific growth rate (µmax) has been found to be 0.0048 h^-1, the half velocity constant (Ks) was 0.0079 gL^-1, and the inhibition growth rate coefficient (Ki) was 0.2619

Original language	English
Pages (from-to)	248-252
Number of pages	5
Journal	Energy Procedia
Volume	153
DOIs	https://doi.org/10.1016/j.egypro.2018.10.015
Publication status	Published - 2018
Event	5th International Conference on Energy and Environment Research, ICEER 2018 - Prague, Czech Republic Duration: 23 Jul 2018 → 27 Jul 2018

Keywords

Bacillus subtilis C19
Biodegradation
Kinetic

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10.1016/j.egypro.2018.10.015

Cite this

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title = "Growth Kinetic of Bacillus subtilis for Pyrene biodegradation",

abstract = "Biodegradation of pyrene compound was catalyzed by multi-component enzymes, and the initial dioxygenase gene was used as key Bacillus subtilis C19 enzyme for aromatic ring structure degradation. The bacterial cell growth rates were also analyzed; it was fitted using Monod, Mason-Mallis, Haldane, Andrews, and Aiba kinetics models. The un-competitive inhibition mechanism of high substrate concentration proposed by Andrews model could be best fitted to experimental bacterial growth kinetic data. Further, the maximum specific growth rate (µmax) has been found to be 0.0048 h-1, the half velocity constant (Ks) was 0.0079 gL-1, and the inhibition growth rate coefficient (Ki) was 0.2619",

keywords = "Bacillus subtilis C19, Biodegradation, Kinetic",

author = "Hanif Yuliani and Perdani, {Meka Saima} and Meilani Manurung and Muhamad Sahlan and Anondho Wijanarko and Heri Hermansyah",

note = "Publisher Copyright: {\textcopyright} 2018 The Authors. Published by Elsevier Ltd.; 5th International Conference on Energy and Environment Research, ICEER 2018 ; Conference date: 23-07-2018 Through 27-07-2018",

year = "2018",

doi = "10.1016/j.egypro.2018.10.015",

language = "English",

volume = "153",

pages = "248--252",

journal = "Energy Procedia",

issn = "1876-6102",

publisher = "Elsevier Ltd",

}

TY - JOUR

T1 - Growth Kinetic of Bacillus subtilis for Pyrene biodegradation

AU - Yuliani, Hanif

AU - Perdani, Meka Saima

AU - Manurung, Meilani

AU - Sahlan, Muhamad

AU - Wijanarko, Anondho

AU - Hermansyah, Heri

PY - 2018

Y1 - 2018

N2 - Biodegradation of pyrene compound was catalyzed by multi-component enzymes, and the initial dioxygenase gene was used as key Bacillus subtilis C19 enzyme for aromatic ring structure degradation. The bacterial cell growth rates were also analyzed; it was fitted using Monod, Mason-Mallis, Haldane, Andrews, and Aiba kinetics models. The un-competitive inhibition mechanism of high substrate concentration proposed by Andrews model could be best fitted to experimental bacterial growth kinetic data. Further, the maximum specific growth rate (µmax) has been found to be 0.0048 h-1, the half velocity constant (Ks) was 0.0079 gL-1, and the inhibition growth rate coefficient (Ki) was 0.2619

AB - Biodegradation of pyrene compound was catalyzed by multi-component enzymes, and the initial dioxygenase gene was used as key Bacillus subtilis C19 enzyme for aromatic ring structure degradation. The bacterial cell growth rates were also analyzed; it was fitted using Monod, Mason-Mallis, Haldane, Andrews, and Aiba kinetics models. The un-competitive inhibition mechanism of high substrate concentration proposed by Andrews model could be best fitted to experimental bacterial growth kinetic data. Further, the maximum specific growth rate (µmax) has been found to be 0.0048 h-1, the half velocity constant (Ks) was 0.0079 gL-1, and the inhibition growth rate coefficient (Ki) was 0.2619

KW - Bacillus subtilis C19

KW - Biodegradation

KW - Kinetic

UR - http://www.scopus.com/inward/record.url?scp=85057427339&partnerID=8YFLogxK

U2 - 10.1016/j.egypro.2018.10.015

DO - 10.1016/j.egypro.2018.10.015

M3 - Conference article

AN - SCOPUS:85057427339

SN - 1876-6102

VL - 153

SP - 248

EP - 252

JO - Energy Procedia

JF - Energy Procedia

T2 - 5th International Conference on Energy and Environment Research, ICEER 2018

Y2 - 23 July 2018 through 27 July 2018

ER -

Growth Kinetic of Bacillus subtilis for Pyrene biodegradation

Abstract

Keywords

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