Growth Kinetic of Bacillus subtilis for Pyrene biodegradation

Hanif Yuliani, Meka Saima Perdani, Meilani Manurung, Muhamad Sahlan, Anondho Wijanarko, Heri Hermansyah

Research output: Contribution to journalConference articlepeer-review

4 Citations (Scopus)


Biodegradation of pyrene compound was catalyzed by multi-component enzymes, and the initial dioxygenase gene was used as key Bacillus subtilis C19 enzyme for aromatic ring structure degradation. The bacterial cell growth rates were also analyzed; it was fitted using Monod, Mason-Mallis, Haldane, Andrews, and Aiba kinetics models. The un-competitive inhibition mechanism of high substrate concentration proposed by Andrews model could be best fitted to experimental bacterial growth kinetic data. Further, the maximum specific growth rate (µmax) has been found to be 0.0048 h-1, the half velocity constant (Ks) was 0.0079 gL-1, and the inhibition growth rate coefficient (Ki) was 0.2619

Original languageEnglish
Pages (from-to)248-252
Number of pages5
JournalEnergy Procedia
Publication statusPublished - 2018
Event5th International Conference on Energy and Environment Research, ICEER 2018 - Prague, Czech Republic
Duration: 23 Jul 201827 Jul 2018


  • Bacillus subtilis C19
  • Biodegradation
  • Kinetic


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