TY - JOUR
T1 - Growth Kinetic of Bacillus subtilis for Pyrene biodegradation
AU - Yuliani, Hanif
AU - Perdani, Meka Saima
AU - Manurung, Meilani
AU - Sahlan, Muhamad
AU - Wijanarko, Anondho
AU - Hermansyah, Heri
N1 - Funding Information:
The authors of this article express deep sense of gratitude towards Prof. Masafumi Yohda of Life and Bioscience Laboratory (Tokyo University of Agriculture and Technology (TUAT), Tokyo, Japan), Dr. Puspita Lisdiyanti (Research Center for Biotechnology, Indonesian Institute of Science (LIPI)). The authors would like to thank for the support provided by Universitas Indonesia, Ministry of Research, Technology and Higher Education Republic of Indonesia, and for the publication support provided by the United States Agency for International Development desonnia’erssivitasorInSHErroamg)flRcelianesearPc(hcadtiouAerhSuHigsanEbe le roughID)(USAht Scientific Modeling, Application, Research and Training for City-centered Innovation and Technology (SMART CITY) Project, Grant #AID-497-A-1600004, Sub Grant #IIE-00000078-UI-1.
Publisher Copyright:
© 2018 The Authors. Published by Elsevier Ltd.
PY - 2018
Y1 - 2018
N2 - Biodegradation of pyrene compound was catalyzed by multi-component enzymes, and the initial dioxygenase gene was used as key Bacillus subtilis C19 enzyme for aromatic ring structure degradation. The bacterial cell growth rates were also analyzed; it was fitted using Monod, Mason-Mallis, Haldane, Andrews, and Aiba kinetics models. The un-competitive inhibition mechanism of high substrate concentration proposed by Andrews model could be best fitted to experimental bacterial growth kinetic data. Further, the maximum specific growth rate (µmax) has been found to be 0.0048 h-1, the half velocity constant (Ks) was 0.0079 gL-1, and the inhibition growth rate coefficient (Ki) was 0.2619
AB - Biodegradation of pyrene compound was catalyzed by multi-component enzymes, and the initial dioxygenase gene was used as key Bacillus subtilis C19 enzyme for aromatic ring structure degradation. The bacterial cell growth rates were also analyzed; it was fitted using Monod, Mason-Mallis, Haldane, Andrews, and Aiba kinetics models. The un-competitive inhibition mechanism of high substrate concentration proposed by Andrews model could be best fitted to experimental bacterial growth kinetic data. Further, the maximum specific growth rate (µmax) has been found to be 0.0048 h-1, the half velocity constant (Ks) was 0.0079 gL-1, and the inhibition growth rate coefficient (Ki) was 0.2619
KW - Bacillus subtilis C19
KW - Biodegradation
KW - Kinetic
UR - http://www.scopus.com/inward/record.url?scp=85057427339&partnerID=8YFLogxK
U2 - 10.1016/j.egypro.2018.10.015
DO - 10.1016/j.egypro.2018.10.015
M3 - Conference article
AN - SCOPUS:85057427339
SN - 1876-6102
VL - 153
SP - 248
EP - 252
JO - Energy Procedia
JF - Energy Procedia
T2 - 5th International Conference on Energy and Environment Research, ICEER 2018
Y2 - 23 July 2018 through 27 July 2018
ER -