Au-doped ZnO nanoparticles were synthesized using eco-friendly substance of Imperata cylindrica L (ICL) leaf extract by the in-situ process. The function of Imperata cylindrica L leaf extract as a medium for the formation reaction of Au-doped ZnO nanoparticles. Powders of Au-doped ZnO nanoparticles was analyzed using Diffuse Reflectance Spectroscopy (UV-Vis DRS), Fourier Transform Infra-red Spectroscopy (FTIR), X-ray Diffraction (XRD), Scanning Electron Microscopy (SEM), Energy Dispersive X-ray (EDX) and Raman Spectroscopy. UV-Vis DRS showed the value of the band gap of ZnO changed from 3.2 eV to 2.5 eV. FTIR showed a reduced -OH absorption peak at wavenumber of 3429 cm-1, 1182 cm-1 and the vibration at 502 cm-1 indicated that Au-doped ZnO nanoparticles successfully synthesized. XRD showed of Au-doped ZnO nanoparticles has a crystallite size of 21.37 nm and decreasing peaks of crystallinity in the miller index (100). The results of SEM-EDX showed the changes of morphology Au-doped ZnO nanoparticles from hexagonal to sphere with the composition of elements is Zn, O and Au. Raman spectroscopy showed a strong vibration peaks at 320 cm-1, 410 cm-1, 590 cm-1 and 1090 cm-1.