TY - GEN
T1 - GnRH receptor expression and endometrial cell proliferation of Macaca nemestrina after administration of GnRH agonist in controlled ovarian stimulation procedures
AU - Pradhita, Andhea
AU - Sahar, Nurhuda
AU - Natadisastra, Muharram
AU - Dzulhulaifah, Wa Ode
AU - Thuffi, Rosalina
N1 - Publisher Copyright:
© 2019 Author(s).
Copyright:
Copyright 2019 Elsevier B.V., All rights reserved.
PY - 2019/12/10
Y1 - 2019/12/10
N2 - GnRH is widely used in the embryo fertilization program (FIV) as one of the ovarian stimulation regimens. At the implantation window, GnRH agonists are known to have an effect on the endometrium directly or indirectly. GnRH estimated has a negative effect on the development of endometrial cells after ovarian stimulation. This study is to analyze the impact of GnRH agonist on ovarian stimulation procedures on receptor expression and endometrial cell apoptosis. The study sample was a stored biological material (BBT) from serum and the endometrial tissue of Macaca nemestrina. The total sample is 8 and divided into 2 groups, the stimulated and control groups. Each sample will be examined 2 types which are the enzyme-linked immunosorbent assay (ELISA) for serum and immunohistochemistry (IHC) for endometrial tissue. IHC was performed with anti-GnRH and caspase 3 antibody. Concentration is measured using an ELISA reader and then converts Optical Density (OD) to a concentration using SoftMax Pro Software. Calculations of stained and unmarked cells were performed using the Image-J Analyzer programs and scored by HSCORE. There is no significant difference between GnRH serum in stimulation or control group (p=0.773). There was no correlation between GnRH in serum with GnRH receptor in endometrial tissue (p=0.060) and activation of caspase (p=0.193). There was no difference between stimulation group and control group in the amount of GnRH in serum, presence of active GnRHR, and Caspase 3 activation. Keywords: GnRH receptors, Macaca nemestria, GnRH Agonist, Controlled ovarian stimulation.
AB - GnRH is widely used in the embryo fertilization program (FIV) as one of the ovarian stimulation regimens. At the implantation window, GnRH agonists are known to have an effect on the endometrium directly or indirectly. GnRH estimated has a negative effect on the development of endometrial cells after ovarian stimulation. This study is to analyze the impact of GnRH agonist on ovarian stimulation procedures on receptor expression and endometrial cell apoptosis. The study sample was a stored biological material (BBT) from serum and the endometrial tissue of Macaca nemestrina. The total sample is 8 and divided into 2 groups, the stimulated and control groups. Each sample will be examined 2 types which are the enzyme-linked immunosorbent assay (ELISA) for serum and immunohistochemistry (IHC) for endometrial tissue. IHC was performed with anti-GnRH and caspase 3 antibody. Concentration is measured using an ELISA reader and then converts Optical Density (OD) to a concentration using SoftMax Pro Software. Calculations of stained and unmarked cells were performed using the Image-J Analyzer programs and scored by HSCORE. There is no significant difference between GnRH serum in stimulation or control group (p=0.773). There was no correlation between GnRH in serum with GnRH receptor in endometrial tissue (p=0.060) and activation of caspase (p=0.193). There was no difference between stimulation group and control group in the amount of GnRH in serum, presence of active GnRHR, and Caspase 3 activation. Keywords: GnRH receptors, Macaca nemestria, GnRH Agonist, Controlled ovarian stimulation.
KW - Controlled ovarian stimulation
KW - GnRH agonist
KW - GnRH receptors
UR - http://www.scopus.com/inward/record.url?scp=85076730903&partnerID=8YFLogxK
U2 - 10.1063/1.5139368
DO - 10.1063/1.5139368
M3 - Conference contribution
AN - SCOPUS:85076730903
T3 - AIP Conference Proceedings
BT - 4th Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, Health, and Medical Devices
A2 - Lischer, Kenny
A2 - Abuzairi, Tomy
A2 - Rahman, Siti Fauziyah
A2 - Gozan, Misri
PB - American Institute of Physics Inc.
T2 - 4th International Symposium of Biomedical Engineering�s Recent Progress in Biomaterials, Drugs Development, Health, and Medical Devices, ISBE 2019
Y2 - 22 July 2019 through 24 July 2019
ER -