Genotyping of Toxoplasma gondii in Cerebral and Ocular Toxoplasmosis

Dearikha Karina Mayashinta; Ryan Halleyantoro; Ika Puspa Sari; Agnes Kurniawan

doi:10.11594/jtls.08.03.02

Genotyping of Toxoplasma gondii in Cerebral and Ocular Toxoplasmosis

Dearikha Karina Mayashinta, Ryan Halleyantoro, Ika Puspa Sari, Agnes Kurniawan

Department of Parasitology Pre Clinic

Research output: Contribution to journal › Article › peer-review

Abstract

Toxoplasma gondii is an obligate intracellular protozoon, spread world-wide and capable of infecting birds and mammals. Genetic information on T. gondii type that causes human toxoplasmosis is limited. In this study, genetic analysis of SAG2 locus was used to determine the genotype of T. gondii from cases with cerebral and ocular toxoplasmosis in Indonesia. Genotype determination was done directly on the clinical samples. A number of 28 cerebrospinal fluid and 8 vitreous humor positively infected with T. gondii, underwent PCR-RFLP to classify each isolate into one of three genotypes of T. gondii. Type I was the most common found suggesting that cerebral and ocular toxoplasmosis in Indonesia is mostly caused by type I strain of T. gondii.

Original language	English
Pages (from-to)	211-216
Journal	Journal of Tropical Life Science
Volume	8
Issue number	3
DOIs	https://doi.org/10.11594/jtls.08.03.02
Publication status	Published - 1 May 2018

Keywords

Toxoplasma gondii
genotype
PCR-RFLP
cerebrospinal fluid
ocular fluid

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abstract = "Toxoplasma gondii is an obligate intracellular protozoon, spread world-wide and capable of infecting birds and mammals. Genetic information on T. gondii type that causes human toxoplasmosis is limited. In this study, genetic analysis of SAG2 locus was used to determine the genotype of T. gondii from cases with cerebral and ocular toxoplasmosis in Indonesia. Genotype determination was done directly on the clinical samples. A number of 28 cerebrospinal fluid and 8 vitreous humor positively infected with T. gondii, underwent PCR-RFLP to classify each isolate into one of three genotypes of T. gondii. Type I was the most common found suggesting that cerebral and ocular toxoplasmosis in Indonesia is mostly caused by type I strain of T. gondii.",

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Genotyping of Toxoplasma gondii in Cerebral and Ocular Toxoplasmosis

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Keywords

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