Genomic characterization of Zika virus isolated from Indonesia

Frilasita A. Yudhaputri, Hidayat Trimarsanto, Aditya Perkasa, Benediktus Yohan, Sotianingsih Haryanto, Ageng Wiyatno, Amin Subandrio W. Kusumo, Khin Saw Myint, Jeremy P. Ledermann, Ronald Rosenberg, Ann M. Powers, R. Tedjo Sasmono

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6 Citations (Scopus)

Abstract

Zika virus (ZIKV) JMB-185 strain was isolated from a febrile patient in Jambi, Indonesia in 2014. To understand its genetic characteristics, we performed whole genome sequencing using the Ion Torrent PGM platform on the supernatant of the first passage. The phylogenetic analysis showed that the isolate was not closely related to the Brazilian ZIKV associated with microcephaly or isolates from the recent Singapore Zika outbreak. Molecular evolution analysis indicated that JMB-185 strain may have been circulating in the Southeast Asia region, including Indonesia since 2000. We observed high nucleotide sequence identity between Indonesia, Thailand, Singapore, and American strains although unique amino acid substitutions were also observed. This report provides information on the genomic characteristics of Indonesian ZIKV which may be used for further studies.

Original languageEnglish
Pages (from-to)248-251
Number of pages4
JournalVirology
Volume510
DOIs
Publication statusPublished - 1 Oct 2017

Keywords

  • Amino acid substitution
  • Genome
  • Indonesia
  • Zika virus

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    Yudhaputri, F. A., Trimarsanto, H., Perkasa, A., Yohan, B., Haryanto, S., Wiyatno, A., Kusumo, A. S. W., Myint, K. S., Ledermann, J. P., Rosenberg, R., Powers, A. M., & Sasmono, R. T. (2017). Genomic characterization of Zika virus isolated from Indonesia. Virology, 510, 248-251. https://doi.org/10.1016/j.virol.2017.07.026