TY - JOUR
T1 - Genomic characterization of Zika virus isolated from Indonesia
AU - Yudhaputri, Frilasita A.
AU - Trimarsanto, Hidayat
AU - Perkasa, Aditya
AU - Yohan, Benediktus
AU - Haryanto, Sotianingsih
AU - Wiyatno, Ageng
AU - Kusumo, Amin Subandrio W.
AU - Myint, Khin Saw
AU - Ledermann, Jeremy P.
AU - Rosenberg, Ronald
AU - Powers, Ann M.
AU - Sasmono, R. Tedjo
PY - 2017/10/1
Y1 - 2017/10/1
N2 - Zika virus (ZIKV) JMB-185 strain was isolated from a febrile patient in Jambi, Indonesia in 2014. To understand its genetic characteristics, we performed whole genome sequencing using the Ion Torrent PGM platform on the supernatant of the first passage. The phylogenetic analysis showed that the isolate was not closely related to the Brazilian ZIKV associated with microcephaly or isolates from the recent Singapore Zika outbreak. Molecular evolution analysis indicated that JMB-185 strain may have been circulating in the Southeast Asia region, including Indonesia since 2000. We observed high nucleotide sequence identity between Indonesia, Thailand, Singapore, and American strains although unique amino acid substitutions were also observed. This report provides information on the genomic characteristics of Indonesian ZIKV which may be used for further studies.
AB - Zika virus (ZIKV) JMB-185 strain was isolated from a febrile patient in Jambi, Indonesia in 2014. To understand its genetic characteristics, we performed whole genome sequencing using the Ion Torrent PGM platform on the supernatant of the first passage. The phylogenetic analysis showed that the isolate was not closely related to the Brazilian ZIKV associated with microcephaly or isolates from the recent Singapore Zika outbreak. Molecular evolution analysis indicated that JMB-185 strain may have been circulating in the Southeast Asia region, including Indonesia since 2000. We observed high nucleotide sequence identity between Indonesia, Thailand, Singapore, and American strains although unique amino acid substitutions were also observed. This report provides information on the genomic characteristics of Indonesian ZIKV which may be used for further studies.
KW - Amino acid substitution
KW - Genome
KW - Indonesia
KW - Zika virus
UR - http://www.scopus.com/inward/record.url?scp=85026229221&partnerID=8YFLogxK
U2 - 10.1016/j.virol.2017.07.026
DO - 10.1016/j.virol.2017.07.026
M3 - Article
C2 - 28755588
AN - SCOPUS:85026229221
VL - 510
SP - 248
EP - 251
JO - Virology
JF - Virology
SN - 0042-6822
ER -
