Genetic variations and relationship among dengue virus type 3 strains isolated from patients with mild or severe form of dengue disease in Indonesia and Thailand

Muhareva Raekiansyah, Andri Pramesyanti, Budiman Bela, Herman Kosasih, Chairin N. Ma'roef, Sherley Yurike Tobing, Panji I. Rudiman, Bachti Alisjahbana, Timothy P. Endi, Sharone Green, Siripen Kalayanarooj, Alan L. Rothman, Tjahjani Mirawati Sudiro

Research output: Contribution to journalArticlepeer-review

17 Citations (Scopus)

Abstract

Sequence analysis was conducted on structural and non-structural genes of 7 strains of dengue virus type-3 (DENV-3 virus) isolated in Indonesia and Thailand in the year 1973, 1994, and 1998 from patients with different clinical manifestations. In general, sequence similarity among isolates was greater than 93%, indicating that the mutation rate of DENV-3 circulating in this region was not more than 7% in the last 3 decades and suggesting that sequences that may responsible for viral architectures and/or biological function were strictly conserved. Mutations unique to viral strains associated with specific clinical manifestations were not found. Alignment of PrM/M and E nucleic acid sequences followed by parsimony analysis of sequences obtained in this study and published elsewhere allowed generation of phylogenetic trees, demonstrating that DENV-3 strains isolated in Indonesia in 1998 belonged to a separate cluster (subtype 2) from those isolated between 1973-1985 (subtype 1).

Original languageEnglish
Pages (from-to)1187-1197
Number of pages11
JournalSoutheast Asian Journal of Tropical Medicine and Public Health
Volume36
Issue number5
Publication statusPublished - 1 Sep 2005

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