TY - JOUR
T1 - Genetic variations and relationship among dengue virus type 3 strains isolated from patients with mild or severe form of dengue disease in Indonesia and Thailand
AU - Raekiansyah, Muhareva
AU - Pramesyanti, Andri
AU - Bela, Budiman
AU - Kosasih, Herman
AU - Ma'roef, Chairin N.
AU - Tobing, Sherley Yurike
AU - Rudiman, Panji I.
AU - Alisjahbana, Bachti
AU - Endi, Timothy P.
AU - Green, Sharone
AU - Kalayanarooj, Siripen
AU - Rothman, Alan L.
AU - Sudiro, T. Mirawati
PY - 2005/9
Y1 - 2005/9
N2 - Sequence analysis was conducted on structural and non-structural genes of 7 strains of dengue virus type-3 (DENV-3 virus) isolated in Indonesia and Thailand in the year 1973, 1994, and 1998 from patients with different clinical manifestations. In general, sequence similarity among isolates was greater than 93%, indicating that the mutation rate of DENV-3 circulating in this region was not more than 7% in the last 3 decades and suggesting that sequences that may responsible for viral architectures and/or biological function were strictly conserved. Mutations unique to viral strains associated with specific clinical manifestations were not found. Alignment of PrM/M and E nucleic acid sequences followed by parsimony analysis of sequences obtained in this study and published elsewhere allowed generation of phylogenetic trees, demonstrating that DENV-3 strains isolated in Indonesia in 1998 belonged to a separate cluster (subtype 2) from those isolated between 1973-1985 (subtype 1).
AB - Sequence analysis was conducted on structural and non-structural genes of 7 strains of dengue virus type-3 (DENV-3 virus) isolated in Indonesia and Thailand in the year 1973, 1994, and 1998 from patients with different clinical manifestations. In general, sequence similarity among isolates was greater than 93%, indicating that the mutation rate of DENV-3 circulating in this region was not more than 7% in the last 3 decades and suggesting that sequences that may responsible for viral architectures and/or biological function were strictly conserved. Mutations unique to viral strains associated with specific clinical manifestations were not found. Alignment of PrM/M and E nucleic acid sequences followed by parsimony analysis of sequences obtained in this study and published elsewhere allowed generation of phylogenetic trees, demonstrating that DENV-3 strains isolated in Indonesia in 1998 belonged to a separate cluster (subtype 2) from those isolated between 1973-1985 (subtype 1).
UR - http://www.scopus.com/inward/record.url?scp=30344449504&partnerID=8YFLogxK
M3 - Article
C2 - 16438144
AN - SCOPUS:30344449504
SN - 0125-1562
VL - 36
SP - 1187
EP - 1197
JO - Southeast Asian Journal of Tropical Medicine and Public Health
JF - Southeast Asian Journal of Tropical Medicine and Public Health
IS - 5
ER -
