Genetic stability of in vitro propagated transgenic Acacia mangium expressing xyloglucanase

N. Utami, S. Hartati, A. Salamah

Research output: Contribution to journalConference article

Abstract

In 2011, a gene transformation experiment in A. mangium was carried out to yield transgenic A. mangium that overexpresses the xyloglucanase gene, enabling plants to grow faster. The transgenic A. mangium has been maintained and continuously subcultured since. Here, we tested the stability of the xyloglucanase gene in transgenic A. mangium following in vitro propagation. Molecular and morphological approaches were used. For all specimens tested, the wild-type A. mangium (K) and two lines of transgenic A. mangium named X11 and X21 were positive for the xyloglucanase gene after recurring subculture. Morphological observations did not reveal any alterations, and the cultures had undergone notable physiological deterioration.

Original languageEnglish
Article number012024
JournalIOP Conference Series: Earth and Environmental Science
Volume481
Issue number1
DOIs
Publication statusPublished - 27 Apr 2020
EventLife and Environmental Sciences Academics Forum 2018, LEAF 2018 - Depok, West Java, Indonesia
Duration: 1 Nov 2018 → …

Keywords

  • Acacia mangium
  • in vitro
  • tissue culture
  • transgenic

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