TY - JOUR
T1 - Genetic diversity of S.typhi
AU - International Multicentre Study Group on the Molecular Epidemiology & Genetic Diversity of S. typhi
AU - Nair, Satheesh
AU - Puthucheary, S.
AU - Altwegg, M.
AU - Pang, T.
AU - Yassin, R.
AU - Sudarmono, P.
AU - Sudarmono, Pratiwi Pudjilestari
AU - Handojo, I.
AU - Padmidewi, M.
AU - Passey, M.
AU - Combs, B.
AU - Chaudhry, R.
AU - Cordano, A. M.
AU - Dougan, G.
AU - Calva, E.
AU - Groisman, E.
N1 - Publisher Copyright:
© 1998, Faculty of Medicine, Universitas Indonesia. All rights reserved.
PY - 1998
Y1 - 1998
N2 - We studied the genetic diversity within specific genes among S. typhi isolates from different geographic regions using various gene probes (Southern blot hybridization) after restriction endonuclease digestion of genomic DNA. Isolates from Malaysia, Indonesia, Papua New Guinea, India, Chile and Switzerland were studied. Polymorphisms were detected among these isolates when fliC, groEL, 16S rRNA and rfbS gene probes were used to probe restricted DNA. In contrast, homogeneity in hybridization pattern was observed when an ompC gene probe was used. Further testing was performed with gene probes for the 16S-23S rRNA spacer region, viaB, invA, and the pathogenicity island gene cluster for Salmonella spp, SPI and SPII. The present study confirms the significant genetic diversity among S. typhi isolates, not only at the total genomic DNA level, but at the level of specific genes. It would be of interest to determine any correlation between genetic polymorphisms and biological properties, especially those related to virulence.
AB - We studied the genetic diversity within specific genes among S. typhi isolates from different geographic regions using various gene probes (Southern blot hybridization) after restriction endonuclease digestion of genomic DNA. Isolates from Malaysia, Indonesia, Papua New Guinea, India, Chile and Switzerland were studied. Polymorphisms were detected among these isolates when fliC, groEL, 16S rRNA and rfbS gene probes were used to probe restricted DNA. In contrast, homogeneity in hybridization pattern was observed when an ompC gene probe was used. Further testing was performed with gene probes for the 16S-23S rRNA spacer region, viaB, invA, and the pathogenicity island gene cluster for Salmonella spp, SPI and SPII. The present study confirms the significant genetic diversity among S. typhi isolates, not only at the total genomic DNA level, but at the level of specific genes. It would be of interest to determine any correlation between genetic polymorphisms and biological properties, especially those related to virulence.
UR - http://www.scopus.com/inward/record.url?scp=85008599048&partnerID=8YFLogxK
U2 - 10.13181/mji.v7iSupp1.1130
DO - 10.13181/mji.v7iSupp1.1130
M3 - Article
AN - SCOPUS:85008599048
SN - 0853-1773
VL - 7
SP - 264
JO - Medical Journal of Indonesia
JF - Medical Journal of Indonesia
ER -