In patients who use the prosthesis, gait analysis is an activity to observe the pattern of walking use the prosthesis for the patient. This activity aims to obtain information relating to balance, comfort, ease of use and the possibility of disorder experienced by the patient during walking using a prosthesis. This study aims to perform gait analysis of lower limb prosthesis, which was developed from ramie fiber composite materials (RE), especially in the socket component. The methods used in gait analysis is the patient walk on a path that has been determined for 6 minutes for each prosthesis produced. Gait analysis data include steps total, cadence (steps/min), step time (s), right step length (cm), left step length (cm), the step width (cm), stride length (cm), stride total, walking length total (m), walking length total per minute (m), walking speed (m/s). Based on the analysis of test results that have been done, it can be concluded that the prosthesis produced from RE has a good comfort level. Prototype with a socket prosthesis produced of RE is lighter, stronger and more elastic compared with socket produced of FGP and ramie RP. This condition greatly affects the comfort level felt by the respondents.