Functional role of PECAM-1/CD31 molecule expressed on human cord blood progenitors

S. El Marsafy; E. D. Carosella; S. G. Agrawal; E. Gluckman; Indra Gusti Mansur; A. Elhabazi; L. Boumsell; A. Bensussan

Functional role of PECAM-1/CD31 molecule expressed on human cord blood progenitors

S. El Marsafy, E. D. Carosella, S. G. Agrawal, E. Gluckman, Indra Gusti Mansur, A. Elhabazi, L. Boumsell, A. Bensussan

Department of Medical Biology Pre Clinic

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

CD31/PECAM-1 (platelet endothelial cell adhesion molecule-1) is a 130 kDa integral membrane protein of the immunoglobulin gene superfamily with the distinctive feature of being expressed on several cell types associated with the vascular compartment. In the present study we report a novel, unique CD31 mAb termed IP28A which reacts with all CD34 molecule expressing hematopoietic progenitor cells and a subset of T, B and NK lymphocytes from human cord blood. Interestingly, we show that the number of CFU-GM and BFU-E was significantly augmented in cord blood progenitor cultures when purified IP28A mAb was added to rhSCF plus rhGM-CSF and rhEpo, respectively. Thus, these results are of relevance in the field of hematopoietic stem cell transplantation as they reveal an agonistic property of the IP28A/CD31 mAb on the differentiation of cord blood progenitor cells.

Original language	English
Pages (from-to)	1340-1346
Number of pages	7
Journal	Leukemia
Volume	10
Issue number	8
Publication status	Published - Aug 1996

Keywords

Mononuclear cells
Stem cell transplantation
Umbilical cord blood

Cite this

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title = "Functional role of PECAM-1/CD31 molecule expressed on human cord blood progenitors",

abstract = "CD31/PECAM-1 (platelet endothelial cell adhesion molecule-1) is a 130 kDa integral membrane protein of the immunoglobulin gene superfamily with the distinctive feature of being expressed on several cell types associated with the vascular compartment. In the present study we report a novel, unique CD31 mAb termed IP28A which reacts with all CD34 molecule expressing hematopoietic progenitor cells and a subset of T, B and NK lymphocytes from human cord blood. Interestingly, we show that the number of CFU-GM and BFU-E was significantly augmented in cord blood progenitor cultures when purified IP28A mAb was added to rhSCF plus rhGM-CSF and rhEpo, respectively. Thus, these results are of relevance in the field of hematopoietic stem cell transplantation as they reveal an agonistic property of the IP28A/CD31 mAb on the differentiation of cord blood progenitor cells.",

keywords = "Mononuclear cells, Stem cell transplantation, Umbilical cord blood",

author = "{El Marsafy}, S. and Carosella, {E. D.} and Agrawal, {S. G.} and E. Gluckman and Mansur, {Indra Gusti} and A. Elhabazi and L. Boumsell and A. Bensussan",

year = "1996",

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language = "English",

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journal = "Leukemia",

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T1 - Functional role of PECAM-1/CD31 molecule expressed on human cord blood progenitors

AU - El Marsafy, S.

AU - Carosella, E. D.

AU - Agrawal, S. G.

AU - Gluckman, E.

AU - Mansur, Indra Gusti

AU - Elhabazi, A.

AU - Boumsell, L.

AU - Bensussan, A.

PY - 1996/8

Y1 - 1996/8

N2 - CD31/PECAM-1 (platelet endothelial cell adhesion molecule-1) is a 130 kDa integral membrane protein of the immunoglobulin gene superfamily with the distinctive feature of being expressed on several cell types associated with the vascular compartment. In the present study we report a novel, unique CD31 mAb termed IP28A which reacts with all CD34 molecule expressing hematopoietic progenitor cells and a subset of T, B and NK lymphocytes from human cord blood. Interestingly, we show that the number of CFU-GM and BFU-E was significantly augmented in cord blood progenitor cultures when purified IP28A mAb was added to rhSCF plus rhGM-CSF and rhEpo, respectively. Thus, these results are of relevance in the field of hematopoietic stem cell transplantation as they reveal an agonistic property of the IP28A/CD31 mAb on the differentiation of cord blood progenitor cells.

AB - CD31/PECAM-1 (platelet endothelial cell adhesion molecule-1) is a 130 kDa integral membrane protein of the immunoglobulin gene superfamily with the distinctive feature of being expressed on several cell types associated with the vascular compartment. In the present study we report a novel, unique CD31 mAb termed IP28A which reacts with all CD34 molecule expressing hematopoietic progenitor cells and a subset of T, B and NK lymphocytes from human cord blood. Interestingly, we show that the number of CFU-GM and BFU-E was significantly augmented in cord blood progenitor cultures when purified IP28A mAb was added to rhSCF plus rhGM-CSF and rhEpo, respectively. Thus, these results are of relevance in the field of hematopoietic stem cell transplantation as they reveal an agonistic property of the IP28A/CD31 mAb on the differentiation of cord blood progenitor cells.

KW - Mononuclear cells

KW - Stem cell transplantation

KW - Umbilical cord blood

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M3 - Article

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AN - SCOPUS:0029763493

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JO - Leukemia

JF - Leukemia

IS - 8

ER -

Functional role of PECAM-1/CD31 molecule expressed on human cord blood progenitors

Abstract

Keywords

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