Abstract
The O6-methylguanine DNA methyltransferase (MGMT) gene encodes a DNA repair protein and plays a role in embryonic development, including the processes leading to congenital malformations such as orofacial cleft. Methylation at CpG islands of the MGMT gene promoter can affect the regulation of the expression of MGMT or other genes. The aim of this study was to investigate the methylation frequency of the MGMT gene in patients with orofacial cleft. The methylation status of the MGMT gene promoter was assessed by methylation-specific polymerase chain reaction (MSP) in 24 subjects with orofacial cleft and 24 healthy individuals. The orofacial cleft group consisted of a significantly (p = 0.04) higher fraction of subjects with fully methylated MGMT gene promoter than the healthy control group. The finding of the elevated frequency of the methylated MGMT gene suggests a possible risk of reduced DNA repair ability in patients with orofacial cleft.
| Original language | English |
|---|---|
| Pages (from-to) | 1531-1535 |
| Number of pages | 5 |
| Journal | Journal of International Dental and Medical Research |
| Volume | 13 |
| Issue number | 4 |
| Publication status | Published - 2020 |
Keywords
- Gene methylation
- O6-methylguanine DNA methyltransferase
- orofacial cleft