Fractionation of the N-hexane extract of Garcinia Bancana Miq. (Manggis Hutan) leaves and its antioxidant activity based on 1,1-diphenyl-2- picrylhydrazyl and ferric reducing antioxidant power assays

Objective: To assess the antioxidant activity from another part of the plant, in this study, leaf extracts in n-hexane were fractionated. Methods: Ten fractions were obtained and tested in vitro for antioxidant activity using two methods, 1,1-diphenyl-2-picrylhydrazyl (DPPH) and ferric reducing antioxidant power (FRAP), to identify the most active fraction. Results: The IC₅₀ of the most active fraction was 36.24 µg/mL using the DPPH method, and the EC₅₀ was 39.54 µg/mL using the FRAP method. The most active fraction was also shown to contain terpenoids. Conclusion: The most active fraction of an n-hexane extract of the leaves of Gacinia bancana Miq., which was tested by both DPPH and FRAP methods had antioxidant activities with IC₅₀ and EC₅₀ values of 36.2482 µg/mL and 39.5442 µg/mL, respectively. Phytochemical screening showed that active fraction contains terpenoids.