Fractionation and antioxidant activity potency of the extract of Garcinia lateriflora Blume var. javanica Boerl leaf

Putu Gita Maya Widyaswari Mahayasih, Berna Elya, Muhammad Hanafi

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Garcinia lateriflora leaves extract of the family Guttiferae has been known to have excellent antioxidant activity. The objective of the study was to determine the antioxidant effect of the n-hexane, ethyl acetate and methanol extracts of G. lateriflora leaves extract. The antioxidant activity was determined by 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging methods and Feric Reducing Antioxidant Power (FRAP) to determine the antioxidant properties. The extracts were fractionated by using column chromatography. The Methanol extract exhibited the strongest antioxidant activity with EC50 values are 13.95 and 19.65 μg/mL by DPPH and FRAP methods respectively. E13 fraction was the most active fraction from ethyl acetate extract with EC50 value for DPPH scavenging method was 37.14 μg/mL and 34.46 μg/mL for reducing power by the FRAP method. Meanwhile M3 fraction was the most active fraction in methanol extract with EC50 value for DPPH scavenging method was 50.02 μg/mL and 37.32 μg/mL for reducing power by the FRAP method.

Original languageEnglish
Title of host publication2nd Biomedical Engineering�s Recent Progress in Biomaterials, Drugs Development, and Medical Devices
Subtitle of host publicationProceedings of the International Symposium of Biomedical Engineering, ISBE 2017
EditorsRadon Dhelika, Yudan Whulanza, Ghiska Ramahdita, Praswasti P.D.K. Wulan
PublisherAmerican Institute of Physics Inc.
ISBN (Electronic)9780735416253
DOIs
Publication statusPublished - 13 Feb 2018
Event2nd Biomedical Engineering's Recent Progress in Biomaterials, Drugs Development, and Medical Devices, ISBE 2017 - Bali, Indonesia
Duration: 25 Jul 201726 Jul 2017

Publication series

NameAIP Conference Proceedings
Volume1933
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616

Conference

Conference2nd Biomedical Engineering's Recent Progress in Biomaterials, Drugs Development, and Medical Devices, ISBE 2017
CountryIndonesia
CityBali
Period25/07/1726/07/17

Keywords

  • Antioxidant
  • DPPH
  • FRAP
  • Garcinia lateriflora
  • Methanol extract

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    Mahayasih, P. G. M. W., Elya, B., & Hanafi, M. (2018). Fractionation and antioxidant activity potency of the extract of Garcinia lateriflora Blume var. javanica Boerl leaf. In R. Dhelika, Y. Whulanza, G. Ramahdita, & P. P. D. K. Wulan (Eds.), 2nd Biomedical Engineering�s Recent Progress in Biomaterials, Drugs Development, and Medical Devices: Proceedings of the International Symposium of Biomedical Engineering, ISBE 2017 [030018] (AIP Conference Proceedings; Vol. 1933). American Institute of Physics Inc.. https://doi.org/10.1063/1.5023965