Fractionation and antioxidant activity potency of the extract of Garcinia lateriflora Blume var. javanica Boerl leaf

Putu Gita Maya Widyaswari Mahayasih; Berna Elya; Muhammad Hanafi

doi:10.1063/1.5023965

Fractionation and antioxidant activity potency of the extract of Garcinia lateriflora Blume var. javanica Boerl leaf

Putu Gita Maya Widyaswari Mahayasih, Berna Elya, Muhammad Hanafi

Department of Pharmacy

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

4 Citations (Scopus)

Abstract

Garcinia lateriflora leaves extract of the family Guttiferae has been known to have excellent antioxidant activity. The objective of the study was to determine the antioxidant effect of the n-hexane, ethyl acetate and methanol extracts of G. lateriflora leaves extract. The antioxidant activity was determined by 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging methods and Feric Reducing Antioxidant Power (FRAP) to determine the antioxidant properties. The extracts were fractionated by using column chromatography. The Methanol extract exhibited the strongest antioxidant activity with EC₅₀ values are 13.95 and 19.65 μg/mL by DPPH and FRAP methods respectively. E13 fraction was the most active fraction from ethyl acetate extract with EC₅₀ value for DPPH scavenging method was 37.14 μg/mL and 34.46 μg/mL for reducing power by the FRAP method. Meanwhile M3 fraction was the most active fraction in methanol extract with EC₅₀ value for DPPH scavenging method was 50.02 μg/mL and 37.32 μg/mL for reducing power by the FRAP method.

Original language	English
Title of host publication	2nd Biomedical Engineering�s Recent Progress in Biomaterials, Drugs Development, and Medical Devices
Subtitle of host publication	Proceedings of the International Symposium of Biomedical Engineering, ISBE 2017
Editors	Radon Dhelika, Yudan Whulanza, Ghiska Ramahdita, Praswasti P.D.K. Wulan
Publisher	American Institute of Physics Inc.
ISBN (Electronic)	9780735416253
DOIs	https://doi.org/10.1063/1.5023965
Publication status	Published - 13 Feb 2018
Event	2nd Biomedical Engineering's Recent Progress in Biomaterials, Drugs Development, and Medical Devices, ISBE 2017 - Bali, Indonesia Duration: 25 Jul 2017 → 26 Jul 2017

Publication series

Name	AIP Conference Proceedings
Volume	1933
ISSN (Print)	0094-243X
ISSN (Electronic)	1551-7616

Conference

Conference	2nd Biomedical Engineering's Recent Progress in Biomaterials, Drugs Development, and Medical Devices, ISBE 2017
Country/Territory	Indonesia
City	Bali
Period	25/07/17 → 26/07/17

Keywords

Antioxidant
DPPH
FRAP
Garcinia lateriflora
Methanol extract

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10.1063/1.5023965

Cite this

Mahayasih, P. G. M. W., Elya, B., & Hanafi, M. (2018). Fractionation and antioxidant activity potency of the extract of Garcinia lateriflora Blume var. javanica Boerl leaf. In R. Dhelika, Y. Whulanza, G. Ramahdita, & P. P. D. K. Wulan (Eds.), 2nd Biomedical Engineering�s Recent Progress in Biomaterials, Drugs Development, and Medical Devices: Proceedings of the International Symposium of Biomedical Engineering, ISBE 2017 Article 030018 (AIP Conference Proceedings; Vol. 1933). American Institute of Physics Inc.. https://doi.org/10.1063/1.5023965

Mahayasih, Putu Gita Maya Widyaswari ; Elya, Berna ; Hanafi, Muhammad. / Fractionation and antioxidant activity potency of the extract of Garcinia lateriflora Blume var. javanica Boerl leaf. 2nd Biomedical Engineering�s Recent Progress in Biomaterials, Drugs Development, and Medical Devices: Proceedings of the International Symposium of Biomedical Engineering, ISBE 2017. editor / Radon Dhelika ; Yudan Whulanza ; Ghiska Ramahdita ; Praswasti P.D.K. Wulan. American Institute of Physics Inc., 2018. (AIP Conference Proceedings).

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title = "Fractionation and antioxidant activity potency of the extract of Garcinia lateriflora Blume var. javanica Boerl leaf",

abstract = "Garcinia lateriflora leaves extract of the family Guttiferae has been known to have excellent antioxidant activity. The objective of the study was to determine the antioxidant effect of the n-hexane, ethyl acetate and methanol extracts of G. lateriflora leaves extract. The antioxidant activity was determined by 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging methods and Feric Reducing Antioxidant Power (FRAP) to determine the antioxidant properties. The extracts were fractionated by using column chromatography. The Methanol extract exhibited the strongest antioxidant activity with EC50 values are 13.95 and 19.65 μg/mL by DPPH and FRAP methods respectively. E13 fraction was the most active fraction from ethyl acetate extract with EC50 value for DPPH scavenging method was 37.14 μg/mL and 34.46 μg/mL for reducing power by the FRAP method. Meanwhile M3 fraction was the most active fraction in methanol extract with EC50 value for DPPH scavenging method was 50.02 μg/mL and 37.32 μg/mL for reducing power by the FRAP method.",

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author = "Mahayasih, {Putu Gita Maya Widyaswari} and Berna Elya and Muhammad Hanafi",

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Mahayasih, PGMW, Elya, B & Hanafi, M 2018, Fractionation and antioxidant activity potency of the extract of Garcinia lateriflora Blume var. javanica Boerl leaf. in R Dhelika, Y Whulanza, G Ramahdita & PPDK Wulan (eds), 2nd Biomedical Engineering�s Recent Progress in Biomaterials, Drugs Development, and Medical Devices: Proceedings of the International Symposium of Biomedical Engineering, ISBE 2017., 030018, AIP Conference Proceedings, vol. 1933, American Institute of Physics Inc., 2nd Biomedical Engineering's Recent Progress in Biomaterials, Drugs Development, and Medical Devices, ISBE 2017, Bali, Indonesia, 25/07/17. https://doi.org/10.1063/1.5023965

Fractionation and antioxidant activity potency of the extract of Garcinia lateriflora Blume var. javanica Boerl leaf. / Mahayasih, Putu Gita Maya Widyaswari; Elya, Berna; Hanafi, Muhammad.
2nd Biomedical Engineering�s Recent Progress in Biomaterials, Drugs Development, and Medical Devices: Proceedings of the International Symposium of Biomedical Engineering, ISBE 2017. ed. / Radon Dhelika; Yudan Whulanza; Ghiska Ramahdita; Praswasti P.D.K. Wulan. American Institute of Physics Inc., 2018. 030018 (AIP Conference Proceedings; Vol. 1933).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

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T1 - Fractionation and antioxidant activity potency of the extract of Garcinia lateriflora Blume var. javanica Boerl leaf

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AU - Elya, Berna

AU - Hanafi, Muhammad

PY - 2018/2/13

Y1 - 2018/2/13

N2 - Garcinia lateriflora leaves extract of the family Guttiferae has been known to have excellent antioxidant activity. The objective of the study was to determine the antioxidant effect of the n-hexane, ethyl acetate and methanol extracts of G. lateriflora leaves extract. The antioxidant activity was determined by 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging methods and Feric Reducing Antioxidant Power (FRAP) to determine the antioxidant properties. The extracts were fractionated by using column chromatography. The Methanol extract exhibited the strongest antioxidant activity with EC50 values are 13.95 and 19.65 μg/mL by DPPH and FRAP methods respectively. E13 fraction was the most active fraction from ethyl acetate extract with EC50 value for DPPH scavenging method was 37.14 μg/mL and 34.46 μg/mL for reducing power by the FRAP method. Meanwhile M3 fraction was the most active fraction in methanol extract with EC50 value for DPPH scavenging method was 50.02 μg/mL and 37.32 μg/mL for reducing power by the FRAP method.

AB - Garcinia lateriflora leaves extract of the family Guttiferae has been known to have excellent antioxidant activity. The objective of the study was to determine the antioxidant effect of the n-hexane, ethyl acetate and methanol extracts of G. lateriflora leaves extract. The antioxidant activity was determined by 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging methods and Feric Reducing Antioxidant Power (FRAP) to determine the antioxidant properties. The extracts were fractionated by using column chromatography. The Methanol extract exhibited the strongest antioxidant activity with EC50 values are 13.95 and 19.65 μg/mL by DPPH and FRAP methods respectively. E13 fraction was the most active fraction from ethyl acetate extract with EC50 value for DPPH scavenging method was 37.14 μg/mL and 34.46 μg/mL for reducing power by the FRAP method. Meanwhile M3 fraction was the most active fraction in methanol extract with EC50 value for DPPH scavenging method was 50.02 μg/mL and 37.32 μg/mL for reducing power by the FRAP method.

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PB - American Institute of Physics Inc.

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Mahayasih PGMW, Elya B, Hanafi M. Fractionation and antioxidant activity potency of the extract of Garcinia lateriflora Blume var. javanica Boerl leaf. In Dhelika R, Whulanza Y, Ramahdita G, Wulan PPDK, editors, 2nd Biomedical Engineering�s Recent Progress in Biomaterials, Drugs Development, and Medical Devices: Proceedings of the International Symposium of Biomedical Engineering, ISBE 2017. American Institute of Physics Inc. 2018. 030018. (AIP Conference Proceedings). doi: 10.1063/1.5023965

Fractionation and antioxidant activity potency of the extract of Garcinia lateriflora Blume var. javanica Boerl leaf

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