Formulation, stability test and in vitro penetration study of transethosomal gel containing green tea (Camellia sinensis L. Kuntze) leaves extract

Delly Ramadon, Seshiana Sebti Pramesti, Effionora A

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Objective: The aim of this study was to increase penetration of epigallocatechin gallate (EGCG) from the extract using transethosomal gel. Methods: Transethosomes (TE) formulae were made using thin layer hydration method with different concentration of green tea extract which was equivalent to 1% (F1), 1.5% (F2), and 2% (F3) of EGCG. F1 was the chosen formula to be incorporated into a gel as a transethosomal gel (TEG). A gel containing green tea extract was also made as a control called as non-transethosomal gel (NTEG). A stability test and in vitro penetration study of gels using Franz diffusion cell were performed. Results: F1 was the chosen formula because it had a spherical shape, a particle size of 112.14±2.19 nm, PDI of 0.166±0.03, a zeta potential of- 52.05±1.34 mV, and %EE of 58.06±0.08%. Stability test results showed that TEG more stable than NTEG. The amount of EGCG penetrated from TEG and NTEG were 1391.16±34.89 μg/cm2 and 485.29±14.49 μg/cm2, respectively (p<0.05). The lag time for TEG was around 0.99±0.2 h, while NTEG was 8.69±0.2 h (p<0.05). Conclusion: It can be concluded that transethosomes can improve gel stability and increase the amount of EGCG penetrated through the skin.

Original languageEnglish
Pages (from-to)91-96
Number of pages6
JournalInternational Journal of Applied Pharmaceutics
Volume9
Issue number5
DOIs
Publication statusPublished - 1 Aug 2017

Keywords

  • EGCG
  • Green tea leaves extract
  • In vitro penetration
  • Transethosomal gel
  • Transethosomes

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