Formulation, characterization and in vitro skin penetration of green tea (Camellia sinensis L.) leaves extract-loaded solid lipid nanoparticles

Selvina Dzulhi; Effionora Anwar; Tati Nurhayati

doi:10.7324/JAPS.2018.8809

Formulation, characterization and in vitro skin penetration of green tea (Camellia sinensis L.) leaves extract-loaded solid lipid nanoparticles

Selvina Dzulhi, Effionora Anwar, Tati Nurhayati

Department of Pharmacy

Research output: Contribution to journal › Article › peer-review

17 Citations (Scopus)

Abstract

Purpose: This study aims to formulate and evaluate the characteristics and the skin penetration of green tea leaves extract loaded solid lipid nanoparticles (GT-SLN). Methods: GT-SLN was prepared by solvent emulsificationevaporation method. The properties of SLN were characterized by dynamic light scattering (DLS), transmission electron microscopy (TEM), differential scanning calorimetry (DSC), and in vitro penetration studies were investigated with Franz diffusion cells. Results: The GT-SLN1 presented spherical shape with an average particle size of 155.6 ± 10.04 nm; polydispersity index of 0.409 ± 0.05; zeta potential of -30.967 ± 1.29; entrapment efficiency of 81.124 ± 0.443%. In vitro penetration study with Franz diffusion cell showed flux value from GT-SLN1 of 1.965 ± 0.025 μg/cm².hour and GT 0.261 ± 0.005 μg/cm².hour. Conclusion: In vitro skin penetration study showed that solid lipid nanoparticles improved the penetration of epigallocatechin gallate (EGCG) through the stratum corneum.

Original language	English
Pages (from-to)	57-62
Number of pages	6
Journal	Journal of Applied Pharmaceutical Science
Volume	8
Issue number	8
DOIs	https://doi.org/10.7324/JAPS.2018.8809
Publication status	Published - 1 Aug 2018

Keywords

Camellia sinensis
Epigallocatechin gallate (EGCG)
Green tea
Skin penetration
Solid lipid nanoparticle (SLN)
Solvent emulsification

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10.7324/JAPS.2018.8809

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title = "Formulation, characterization and in vitro skin penetration of green tea (Camellia sinensis L.) leaves extract-loaded solid lipid nanoparticles",

abstract = "Purpose: This study aims to formulate and evaluate the characteristics and the skin penetration of green tea leaves extract loaded solid lipid nanoparticles (GT-SLN). Methods: GT-SLN was prepared by solvent emulsificationevaporation method. The properties of SLN were characterized by dynamic light scattering (DLS), transmission electron microscopy (TEM), differential scanning calorimetry (DSC), and in vitro penetration studies were investigated with Franz diffusion cells. Results: The GT-SLN1 presented spherical shape with an average particle size of 155.6 ± 10.04 nm; polydispersity index of 0.409 ± 0.05; zeta potential of -30.967 ± 1.29; entrapment efficiency of 81.124 ± 0.443%. In vitro penetration study with Franz diffusion cell showed flux value from GT-SLN1 of 1.965 ± 0.025 μg/cm2.hour and GT 0.261 ± 0.005 μg/cm2.hour. Conclusion: In vitro skin penetration study showed that solid lipid nanoparticles improved the penetration of epigallocatechin gallate (EGCG) through the stratum corneum.",

keywords = "Camellia sinensis, Epigallocatechin gallate (EGCG), Green tea, Skin penetration, Solid lipid nanoparticle (SLN), Solvent emulsification",

author = "Selvina Dzulhi and Effionora Anwar and Tati Nurhayati",

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year = "2018",

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doi = "10.7324/JAPS.2018.8809",

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AU - Anwar, Effionora

AU - Nurhayati, Tati

PY - 2018/8/1

Y1 - 2018/8/1

N2 - Purpose: This study aims to formulate and evaluate the characteristics and the skin penetration of green tea leaves extract loaded solid lipid nanoparticles (GT-SLN). Methods: GT-SLN was prepared by solvent emulsificationevaporation method. The properties of SLN were characterized by dynamic light scattering (DLS), transmission electron microscopy (TEM), differential scanning calorimetry (DSC), and in vitro penetration studies were investigated with Franz diffusion cells. Results: The GT-SLN1 presented spherical shape with an average particle size of 155.6 ± 10.04 nm; polydispersity index of 0.409 ± 0.05; zeta potential of -30.967 ± 1.29; entrapment efficiency of 81.124 ± 0.443%. In vitro penetration study with Franz diffusion cell showed flux value from GT-SLN1 of 1.965 ± 0.025 μg/cm2.hour and GT 0.261 ± 0.005 μg/cm2.hour. Conclusion: In vitro skin penetration study showed that solid lipid nanoparticles improved the penetration of epigallocatechin gallate (EGCG) through the stratum corneum.

AB - Purpose: This study aims to formulate and evaluate the characteristics and the skin penetration of green tea leaves extract loaded solid lipid nanoparticles (GT-SLN). Methods: GT-SLN was prepared by solvent emulsificationevaporation method. The properties of SLN were characterized by dynamic light scattering (DLS), transmission electron microscopy (TEM), differential scanning calorimetry (DSC), and in vitro penetration studies were investigated with Franz diffusion cells. Results: The GT-SLN1 presented spherical shape with an average particle size of 155.6 ± 10.04 nm; polydispersity index of 0.409 ± 0.05; zeta potential of -30.967 ± 1.29; entrapment efficiency of 81.124 ± 0.443%. In vitro penetration study with Franz diffusion cell showed flux value from GT-SLN1 of 1.965 ± 0.025 μg/cm2.hour and GT 0.261 ± 0.005 μg/cm2.hour. Conclusion: In vitro skin penetration study showed that solid lipid nanoparticles improved the penetration of epigallocatechin gallate (EGCG) through the stratum corneum.

KW - Camellia sinensis

KW - Epigallocatechin gallate (EGCG)

KW - Green tea

KW - Skin penetration

KW - Solid lipid nanoparticle (SLN)

KW - Solvent emulsification

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Formulation, characterization and in vitro skin penetration of green tea (Camellia sinensis L.) leaves extract-loaded solid lipid nanoparticles

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