TY - JOUR
T1 - Formulation, characterization and in vitro skin penetration of green tea (Camellia sinensis L.) leaves extract-loaded solid lipid nanoparticles
AU - Dzulhi, Selvina
AU - Anwar, Effionora
AU - Nurhayati, Tati
N1 - Funding Information:
This research is financially supported by a research grant from National Agency of Drug and Food Control of Republic of Indonesia and Faculty of Pharmacy Universitas Indonesia.
Publisher Copyright:
© 2018 Selvina Dzulhi et al.
PY - 2018/8/1
Y1 - 2018/8/1
N2 - Purpose: This study aims to formulate and evaluate the characteristics and the skin penetration of green tea leaves extract loaded solid lipid nanoparticles (GT-SLN). Methods: GT-SLN was prepared by solvent emulsificationevaporation method. The properties of SLN were characterized by dynamic light scattering (DLS), transmission electron microscopy (TEM), differential scanning calorimetry (DSC), and in vitro penetration studies were investigated with Franz diffusion cells. Results: The GT-SLN1 presented spherical shape with an average particle size of 155.6 ± 10.04 nm; polydispersity index of 0.409 ± 0.05; zeta potential of -30.967 ± 1.29; entrapment efficiency of 81.124 ± 0.443%. In vitro penetration study with Franz diffusion cell showed flux value from GT-SLN1 of 1.965 ± 0.025 μg/cm2.hour and GT 0.261 ± 0.005 μg/cm2.hour. Conclusion: In vitro skin penetration study showed that solid lipid nanoparticles improved the penetration of epigallocatechin gallate (EGCG) through the stratum corneum.
AB - Purpose: This study aims to formulate and evaluate the characteristics and the skin penetration of green tea leaves extract loaded solid lipid nanoparticles (GT-SLN). Methods: GT-SLN was prepared by solvent emulsificationevaporation method. The properties of SLN were characterized by dynamic light scattering (DLS), transmission electron microscopy (TEM), differential scanning calorimetry (DSC), and in vitro penetration studies were investigated with Franz diffusion cells. Results: The GT-SLN1 presented spherical shape with an average particle size of 155.6 ± 10.04 nm; polydispersity index of 0.409 ± 0.05; zeta potential of -30.967 ± 1.29; entrapment efficiency of 81.124 ± 0.443%. In vitro penetration study with Franz diffusion cell showed flux value from GT-SLN1 of 1.965 ± 0.025 μg/cm2.hour and GT 0.261 ± 0.005 μg/cm2.hour. Conclusion: In vitro skin penetration study showed that solid lipid nanoparticles improved the penetration of epigallocatechin gallate (EGCG) through the stratum corneum.
KW - Camellia sinensis
KW - Epigallocatechin gallate (EGCG)
KW - Green tea
KW - Skin penetration
KW - Solid lipid nanoparticle (SLN)
KW - Solvent emulsification
UR - http://www.scopus.com/inward/record.url?scp=85052755801&partnerID=8YFLogxK
U2 - 10.7324/JAPS.2018.8809
DO - 10.7324/JAPS.2018.8809
M3 - Article
AN - SCOPUS:85052755801
SN - 2231-3354
VL - 8
SP - 57
EP - 62
JO - Journal of Applied Pharmaceutical Science
JF - Journal of Applied Pharmaceutical Science
IS - 8
ER -