TY - JOUR
T1 - FORMULATION AND EVALUATION OF TWO CELASTROL NANOEMULSIONS PREPARED FROM TWO OILS
T2 - ISOPROPYL MYRISTATE AND VIRGIN COCONUT OIL
AU - Abdullah, Nur Alam
AU - Jufri, Mahdi
AU - Mun’im, Abdul
AU - Saputri, Fadlina Chany
N1 - Funding Information:
The authors gratefully acknowledge the Directorate of Research and Community Engagements of Universitas Indonesia for financial support: Hibah PUTTI TADOK NKB-3190/UN2. RST/HKP.05.00/ 2020.
Publisher Copyright:
© 2022 The Authors. Published by Innovare Academic Sciences Pvt Ltd. T.
PY - 2022/3/1
Y1 - 2022/3/1
N2 - Objective: Celastrol, which is classified as BCS 4, needs to be developed into a nanoemulsion formula for a stable and good formulation. The aim of the study was to determine the in vitro penetration ability and adsorption efficiency (EE) between two different base oils, namely Isopropyl myristate (IPM) and virgin coconut. oil (VCO). Methods: Two celastrol nanoemulsion formulas were prepared by high energy method using High share homogenizer (HSH) at 15,000 rpm for 15 min, using different oil-based components, F1 IPM and F2 VCO. Particle size, polydispersity index (PDI), D90, zeta potential, and morphology of nanoemulsions was evaluated. In vitro studies by Franz diffusion cell test method determined the difference. Results: The results showed that celastrol can be formulated well with a ternary ratio of 5:45:50 for IPM and 20:30:50 for VCO. The absorption efficiency test for celestrol levels was 96.49%±2.72 for IPM and 76.53%±1.19 for VCO. The mean particle size, PDI, and zeta potential were 70.81±0.20 nm, 0.1±0.03, and 50.2±0.60 mV, for VCO and 186.23±3, respectively. 12 nm, 0.2±0.07, and 45.5±1.10 mV for HDI. Spherical morphology<200 nm. Franz diffusion in vitro at 20 and 24 h, celastrol is well penetrated at levels of 2.4 g/ml gram and 2.5 g/ml for HDI and at 2.0 g/ml gram and 2.4 g/ml, respectively. ml/gram for VCO. Conclusion: Celastrol was successfully developed into nanoemulsions using IPM or VCO, particle size<200 nm, and stable spherical shape.
AB - Objective: Celastrol, which is classified as BCS 4, needs to be developed into a nanoemulsion formula for a stable and good formulation. The aim of the study was to determine the in vitro penetration ability and adsorption efficiency (EE) between two different base oils, namely Isopropyl myristate (IPM) and virgin coconut. oil (VCO). Methods: Two celastrol nanoemulsion formulas were prepared by high energy method using High share homogenizer (HSH) at 15,000 rpm for 15 min, using different oil-based components, F1 IPM and F2 VCO. Particle size, polydispersity index (PDI), D90, zeta potential, and morphology of nanoemulsions was evaluated. In vitro studies by Franz diffusion cell test method determined the difference. Results: The results showed that celastrol can be formulated well with a ternary ratio of 5:45:50 for IPM and 20:30:50 for VCO. The absorption efficiency test for celestrol levels was 96.49%±2.72 for IPM and 76.53%±1.19 for VCO. The mean particle size, PDI, and zeta potential were 70.81±0.20 nm, 0.1±0.03, and 50.2±0.60 mV, for VCO and 186.23±3, respectively. 12 nm, 0.2±0.07, and 45.5±1.10 mV for HDI. Spherical morphology<200 nm. Franz diffusion in vitro at 20 and 24 h, celastrol is well penetrated at levels of 2.4 g/ml gram and 2.5 g/ml for HDI and at 2.0 g/ml gram and 2.4 g/ml, respectively. ml/gram for VCO. Conclusion: Celastrol was successfully developed into nanoemulsions using IPM or VCO, particle size<200 nm, and stable spherical shape.
KW - Celastrol
KW - IPM
KW - Nanoemulsion
KW - Particle size
KW - VCO
UR - http://www.scopus.com/inward/record.url?scp=85126529477&partnerID=8YFLogxK
U2 - 10.22159/ijap.2022v14i2.43509
DO - 10.22159/ijap.2022v14i2.43509
M3 - Article
AN - SCOPUS:85126529477
SN - 0975-7058
VL - 14
SP - 267
EP - 275
JO - International Journal of Applied Pharmaceutics
JF - International Journal of Applied Pharmaceutics
IS - 2
ER -