Formation of TiO2nanotubular layers on Ti-6Al-4V based dental implants for inhibiting biofilm growth

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Modification of Ti-6Al-4V through electrochemical anodization method has been investigated on the purpose of generating TiO2nanotube arrays (TiNTAs) on the surface of Ti-6Al-4V films. The as-anodized samples were calcined in an atmospheric furnace at various temperatures, in the range of 500-800°C. The evaluation of biofilm inhibition was performed by an in vitro method with Streptococcus mutans as a bacterium model. FE-SEM imaging confirmed the successful formation of TiO2nanotube arrays while XRD results implied a phase transformation from anatase to rutile when the calcination temperature was around 600-650°C with average crystallite size of 18 nm. Calcination temperature is one of determining factors in the adjustment of crystallinity and morphology of TiO2, which in turn affects its capability to suppress biofilm formation. This study revealed that the best sample for biofilm inhibition was calcined at 600°C with a crystallite phase of mostly anatase. This sample managed to improve antibacterial activity of up to five times as compared to the unmodified Ti-6Al-4V. The output of this study is expected to give some insight on a promising alternative for preventing the formation of harmful biofilm on dental implants.

Original languageEnglish
Pages (from-to)1543-1548
Number of pages6
JournalAsian Journal of Chemistry
Issue number7
Publication statusPublished - Jul 2020


  • Biofilms
  • Dental implant
  • Surface modification
  • TiOnanotubes


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