One of important processes on the fabrication of dye-sensitized solar cell (DSSC) is formation of TiO2 thin film. Thickness of TiO2 thin film is needed to be controlled in order to obtain optimum TiO2 thickness. Electrophoresis deposition is simple method for thin film deposition which enable us to control the thickness of thin film. In this work, the formation of TiO2 thin film on indium-doped tin oxide (ITO) glass using electrophoresis deposition is numerically and experimentally studied. We have succeeded to control the thickness of TiO2 thin film by changing of electrophoresis applied voltage and deposition time. In the experiment, TiO2 particles were suspended into isopropyl alcohol solution. Mg(NO3)2 was added to the solution in order to obtain the stable solution for electrophoresis. We found numerically that the Mg(NO 3)2 in the range from 10-6M to 10 -4M, is appropriate condition for the electrophoresis deposition. Using this method, we have fabricated DSSC device and study the effect of TiO2 thickness on DSSCs characteristic. Our results shows that the electrophoresis is not only essential for DSSC application but also for the other devices with a given thickness of TiO2 film.