TY - GEN
T1 - Fluorescence analysis of micro-scale surface modification using ultrafine capillary atmospheric pressure plasma jet for biochip fabrication
AU - Nagatsu, Masaaki
AU - Kinpara, Masahiro
AU - Abuzairi, Tomy
N1 - Publisher Copyright:
© Springer International Publishing AG 2017.
PY - 2017
Y1 - 2017
N2 - In this study, we propose the micro-scale surface functionalization techniques using a maskless, versatile, simple tool, represented by a nano- or micro-capillary atmospheric pressure plasma jet (APPJs). We show the possibility of size-controlled surface functionalization on polymer substrate with amino groups or carboxyl groups by using fluorescent technique. Moreover, we prove the successful connection of biomolecules on the functionalized micro-scale patterns, indicating the possibility to use ultrafine capillary APPJs as versatile tools for biosensing, tissue engineering, and related biomedical applications. With use of this technology, we study the biomolecules patterning onto CNT dot array via ultrafine APPJ for biochip fabrication. An ultrafine APPJ with a micro-capillary was utilized to functionalize amino groups on designated CNT spots. Two-stage plasma treatments, pre-treatment and post-treatment, were conducted to functionalize CNT array by ultrafine APPJ. Biomolecules system, such as biotin-avidin system was employed to assess the feasibility of biomolecule immobilization on CNT. The possibility of this technique for micro-biochip applications was successfully demonstrated by patterning biomolecules onto CNT microarrays.
AB - In this study, we propose the micro-scale surface functionalization techniques using a maskless, versatile, simple tool, represented by a nano- or micro-capillary atmospheric pressure plasma jet (APPJs). We show the possibility of size-controlled surface functionalization on polymer substrate with amino groups or carboxyl groups by using fluorescent technique. Moreover, we prove the successful connection of biomolecules on the functionalized micro-scale patterns, indicating the possibility to use ultrafine capillary APPJs as versatile tools for biosensing, tissue engineering, and related biomedical applications. With use of this technology, we study the biomolecules patterning onto CNT dot array via ultrafine APPJ for biochip fabrication. An ultrafine APPJ with a micro-capillary was utilized to functionalize amino groups on designated CNT spots. Two-stage plasma treatments, pre-treatment and post-treatment, were conducted to functionalize CNT array by ultrafine APPJ. Biomolecules system, such as biotin-avidin system was employed to assess the feasibility of biomolecule immobilization on CNT. The possibility of this technique for micro-biochip applications was successfully demonstrated by patterning biomolecules onto CNT microarrays.
KW - Biochip sensor
KW - Micro-scale patterning
KW - Surface modification
KW - Ultrafine capillary atmospheric pressure plasma jet
UR - http://www.scopus.com/inward/record.url?scp=84989958957&partnerID=8YFLogxK
U2 - 10.1007/978-3-319-46490-9_34
DO - 10.1007/978-3-319-46490-9_34
M3 - Conference contribution
AN - SCOPUS:84989958957
SN - 9783319464893
T3 - Advances in Intelligent Systems and Computing
SP - 247
EP - 254
BT - Recent Global Research and Education
A2 - Jabłoński, Ryszard
A2 - Szewczyk, Roman
PB - Springer Verlag
T2 - 15th International Conference on Global Research and Education, INTER-ACADEMIA 2016
Y2 - 26 September 2016 through 28 September 2016
ER -