Fluorescence analysis of micro-scale surface modification using ultrafine capillary atmospheric pressure plasma jet for biochip fabrication

Masaaki Nagatsu, Masahiro Kinpara, Tomy Abuzairi

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

2 Citations (Scopus)

Abstract

In this study, we propose the micro-scale surface functionalization techniques using a maskless, versatile, simple tool, represented by a nano- or micro-capillary atmospheric pressure plasma jet (APPJs). We show the possibility of size-controlled surface functionalization on polymer substrate with amino groups or carboxyl groups by using fluorescent technique. Moreover, we prove the successful connection of biomolecules on the functionalized micro-scale patterns, indicating the possibility to use ultrafine capillary APPJs as versatile tools for biosensing, tissue engineering, and related biomedical applications. With use of this technology, we study the biomolecules patterning onto CNT dot array via ultrafine APPJ for biochip fabrication. An ultrafine APPJ with a micro-capillary was utilized to functionalize amino groups on designated CNT spots. Two-stage plasma treatments, pre-treatment and post-treatment, were conducted to functionalize CNT array by ultrafine APPJ. Biomolecules system, such as biotin-avidin system was employed to assess the feasibility of biomolecule immobilization on CNT. The possibility of this technique for micro-biochip applications was successfully demonstrated by patterning biomolecules onto CNT microarrays.

Original languageEnglish
Title of host publicationRecent Global Research and Education
Subtitle of host publicationTechnological Challenges - Proceedings of the 15th International Conference on Global Research and Education Inter-Academia 2016
EditorsRyszard Jabłoński, Roman Szewczyk
PublisherSpringer Verlag
Pages247-254
Number of pages8
ISBN (Print)9783319464893
DOIs
Publication statusPublished - 1 Jan 2017
Event15th International Conference on Global Research and Education, INTER-ACADEMIA 2016 - Warsaw, Poland
Duration: 26 Sep 201628 Sep 2016

Publication series

NameAdvances in Intelligent Systems and Computing
Volume519
ISSN (Print)2194-5357

Conference

Conference15th International Conference on Global Research and Education, INTER-ACADEMIA 2016
CountryPoland
CityWarsaw
Period26/09/1628/09/16

Keywords

  • Biochip sensor
  • Micro-scale patterning
  • Surface modification
  • Ultrafine capillary atmospheric pressure plasma jet

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