Stem cell therapy is renowned as one of therapeutic treatments for injury, cancer, or other health issues in regard to tissue regeneration. This method needs special approach because stem cell must be retained in a specific environment that can control its viability and advantages. Microencapsulation is one of them. This study is aimed to propose stem cell microencapsulation using microfluidics chip to enable size control of the encapsulated cells. Microfluidics is chosen because it can control the size of the encapsulated cell. The stem cell will be encapsulated in dual layers to enhance its protection in the human body, in which the first layer is alginate + Calcium and the second layer is PEG (polyethylene glycol). However, this paper will focus on the simulation of cell microencapsulation in flow focusing microfluidics channel since flow focusing microfluidics channel has been proven to generate desired microfluidics droplet as encapsulated cell. Using computational fluids dynamics software, the microfluidics droplet is processed by volume of fluids method with multiphase calculation. The primary phase is water and the second phase is oil. The CFD simulation shows that desired droplet is successfully generated. Therefore, this paper can be the first step of the proposed study in the stem cell dual layers microencapsulation.