Ficus elastica Leaf Extract Effect Toward IL-10 and TNF-α as a Preeclampsia Model on Hypoxia-induced EA.hy926

Chrismis Novalinda Ginting, I. Nyoman Ehrich Lister, Ermi Girsang, Maya Sari Mutia, Yolanda Eliza Putri Lubis, Annisa Amalia, Wahyu Setia Widodo, Dwi Surya Artie, Rizal Rizal, Wahyu Widowati

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Preeclampsia is a pregnancy complication characterized by proteinuria, hypertension, hypoxia as well an increased systemic inflammatory response. It affects 5 to 10% of all births and is a leading factor in fetal and maternal morbidity and death. Ficus elastica L. leaves are known to have natural compounds with anti-inflammatory and antioxidant properties. This research assessed the potential of F. elastica leaf extract (FEE) as anti-inflammatory agent in a hypoxic-induced human endothelial cell line (EA.hy926) as preeclampsia model. F. elastica leaves were extracted using 70% ethanol. Cytotoxic assay of the FEE in different concentrations (3.13 to 100 µg/ml) against EA.hy926 was done using MTS assay. The FEE’s anti-inflammatory activity was measured with ELISA for the TNF-α and IL-10 parameters. The FEE did not cause cytotoxicity to the EA.hy926 cells, with viability above 80% at the highest concentration. The FEE could decrease the pro-inflammatory TNF-α level and increase the IL-10 level as anti-inflammatory markers in the preeclampsia model at significance level p < 0.05. This study suggests that FEE is a safe alternative herbal medicine for preeclampsia treatment.

Original languageEnglish
Pages (from-to)487-496
Number of pages10
JournalJournal of Mathematical and Fundamental Sciences
Volume53
Issue number3
DOIs
Publication statusPublished - 3 Dec 2021

Keywords

  • EA.hy926
  • Ficus elastica
  • hypoxia
  • IL-10
  • preeclampsia
  • TNF-α

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