Fertilization rate of Lukas fish (Puntius bramoides)

P. Pertiwi, Abinawanto, S. Yimastria

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The aim of study was to analyze the fertilization rate of Lukas fish (Puntius bramoides) after stimulated by GnRH analogue. Milt and eggs were collected by hand stripping method. Milt was then diluted with physiological NaCl 0.9%. The ratio of milt and diluents was 1:9. The experimental treatment consisted of four groups, such as: A (normal/control, without dilution), B (10 times dilution), C (100 times dilution), and D (1000 times dilution). Eggs were then fertilized manually, and were calculated the percentage of fertility and hatching rate. The result showed that the highest percentage of fertility and hatching rate at 1000 times dilution (78,92 ± 1,81) % and (72,33 ± 4,56) %, respectively. Anova test expresses the optimum fertility and hatching rate showed significant differences at 1000 times dilution (p<0,05). Water quality parameters observed in the study were water temperature of 23°C - 24°C and pH 5 - 6.

Original languageEnglish
Title of host publicationProceedings of the 3rd International Symposium on Current Progress in Mathematics and Sciences 2017, ISCPMS 2017
EditorsRatna Yuniati, Terry Mart, Ivandini T. Anggraningrum, Djoko Triyono, Kiki A. Sugeng
PublisherAmerican Institute of Physics Inc.
ISBN (Electronic)9780735417410
DOIs
Publication statusPublished - 22 Oct 2018
Event3rd International Symposium on Current Progress in Mathematics and Sciences 2017, ISCPMS 2017 - Bali, Indonesia
Duration: 26 Jul 201727 Jul 2017

Publication series

NameAIP Conference Proceedings
Volume2023
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616

Conference

Conference3rd International Symposium on Current Progress in Mathematics and Sciences 2017, ISCPMS 2017
CountryIndonesia
CityBali
Period26/07/1727/07/17

Keywords

  • GnRH analogue
  • Puntius bramoides
  • fertility rate
  • hatching rate

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