Fertilization Rate of Giant Grouper (Epinephelus lanceolatus, Bloch 1790) Sperm Post-cryopreservation and Application on Hybridization in Different Grouper Species

Fertilization was doing to measure the success of sperm cryopreservation because it was more informative. Sperm cryopreservation was expected to get sperm that successfully fertilize the egg well. The aim of this study was to determine the sperm fertilization ability of giant grouper (Epinephelus lanceolatus, Bloch 1790) 48 hours post-cryopreservation in fertilizing tiger grouper eggs (Epinephelus fuscoguttatus, Forskal 1775). Fertilization was carried out artificially using cryopreservation sperm with 6% of glycerol and palm dates concentrates (0%; 5%; 10%; 15%; 20%; and 25%). The tiger grouper brood was previously injected with hormone chorionic gonadotropin at a dose of 500 IU/kg. The results showed that there were significant differences (P < 0.05) on fertilization of tiger grouper eggs with sperm of giant grouper which were cryopreserved with 6% of glycerol and various concentrations of palm date concentrates. Sperm which was cryopreserved with 6% of glycerol and 10% of palm date concentrate has the best fertility ability by showing the highest value of 66.25 ± 3.23%.