Fermentation of antimicrobial substances of Streptomyces sp. BCy isolated from seagrass Cymodocearotundata using two different media

V. Damayanti, R. N. Rachma, Iman Santoso, Yasman, Andi Eko Maryanto

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

ActinomycetesBCy has been isolated from Seagrass Cymodocearotundata, Prapat Agung Coastal Bali Barat, Indonesia. Identification by 16S rRNA showed that the colony belongs to genus Streptomyces. The isolate was grown in two different broth media, Bushnell-Haas (BH) and Production Medium 4 (PM4) for 1 and two weeks. The medium was filtred and dry weight of biomass was measured. Filtrate was extracted using ethyl acetate and also measured for dry weight. Based on dry weight of biomass within two weeks, it showed that Streptomyces sp.BCy grew better in PM4 (700 mg/100 mL) compare to BH (166.8 mg/100 mL). Weight of crude extract in PM4 (15.6 mg/100 mL) also higher than the weight in BH (4.3 mg/100 mL). Antimicrobial activities of crude extract were carried out by Kirby Bauer method and determined by diameter of clear zone. The antimicrobial activity of crude extract more effective in two weeks and were able to inhibit Staphylococcus aureus NBRC 100910 and Candida albicans UICC Y-29.

Original languageEnglish
Title of host publicationProceedings of the 3rd International Symposium on Current Progress in Mathematics and Sciences 2017, ISCPMS 2017
EditorsRatna Yuniati, Terry Mart, Ivandini T. Anggraningrum, Djoko Triyono, Kiki A. Sugeng
PublisherAmerican Institute of Physics Inc.
ISBN (Electronic)9780735417410
DOIs
Publication statusPublished - 22 Oct 2018
Event3rd International Symposium on Current Progress in Mathematics and Sciences 2017, ISCPMS 2017 - Bali, Indonesia
Duration: 26 Jul 201727 Jul 2017

Publication series

NameAIP Conference Proceedings
Volume2023
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616

Conference

Conference3rd International Symposium on Current Progress in Mathematics and Sciences 2017, ISCPMS 2017
Country/TerritoryIndonesia
CityBali
Period26/07/1727/07/17

Keywords

  • Antimicrobial activity
  • Cymodocearotundata
  • Streptomyces sp. BCy
  • crude extract

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