Fecal pregnanediol glucuronide and serum progesterone patterns for ovarian function assessment of Javan Gibbons (Hylobates moloch)

Hera Maheshwari, Luthfiralda Sjahfirdi, Pudji Astuti, Bambang Purwantara, Hadi Sukadi Alikodra, Dondin Sajuthi, Reviany Widjajakusuma

Research output: Contribution to journalArticle

Abstract

Pregnanediol glucuronide (PdG) in feces and serum progesterone (P) were analyzed for determining 4 ovarian cycle as well as to evaluate ovarian function of captive-housed Javan Gibbon (Hylobates moloch). A total of four females Javan Gibbons maintained in single-typed cage at Ragunan Zoo (Owa1 and Owa2) and in pairing-typed cage at Taman Margasatwa Taman Sari, Bandung (Owa3 and Owa4) were used in this research. Fecal and serum samples were collected twice a week throughout 3-months period. Following methanolic extraction of lyophilized fecal powder, samples were analyzed using enzyme linked immunosorbent assay (ELISA) for PdG as well as for P 4 Results indicated that the two females in pairing-typed cage did not show any 4 regular cycle whereas the other two females in single-typed cage showed distinct pattern reflecting cyclicity. Fecal PdG concentrations were ranged between 0.56-4.95 ug/mg DW while the levels of serum P were ranged 4 between 0.09-15.92 ng/ml for all females. The length of the cycle obtained was ranged between 24 and 29 days which comprised of 14-15 days of follicular phase and 10-14 days of luteal phase. The time lag between serum P 4 and PdG excretion was estimated about 6 days.

Original languageEnglish
Pages (from-to)737-744
Number of pages8
JournalWorld Applied Sciences Journal
Volume18
Issue number6
DOIs
Publication statusPublished - 27 Aug 2012

Keywords

  • Fecal Pdg
  • Javan Gibbon
  • Ovarian Cycle
  • Serum Progesterone

Fingerprint Dive into the research topics of 'Fecal pregnanediol glucuronide and serum progesterone patterns for ovarian function assessment of Javan Gibbons (Hylobates moloch)'. Together they form a unique fingerprint.

  • Cite this