Demand for fossil fuel of coal, petroleum and natural gas has always increased from year to year. Therefore, the development, expansion and utilization of biomass from non no-fossil fuels need be carried out. Efforts to explore, develop, process and utilize biomass from non-fossil fuels, which is the cultivation of microalgae containing triglycerides that can be extracted into methyl esters (biodiesel). The sample extraction process is carried out in 5 stages, such as harvesting, weaving, methylation, extraction, and washing. The amount and ratio of saturated and unsaturated fatty acid is a key that determines the suitability of microalgae as a biofuel feedstock. This paper aims to compare the potential microalgae in Indonesia with the other countries as feedstock for biofuel production. The fatty acid composition of Synechococcus sp. HS-9, Spirulina platensis, Glagah consortium microalgae, and Nostoc HS-20 from Indonesia could be a promising feedstock for biofuel production. The quantity of fatty acid microalgae from Indonesia higher than the other countries.