Acute lymphocytic leukemia is a type of leukemia that is most prevalent among children which is around 75-80%. The progress of chemotherapy for acute lymphocytic leukemia has increased the survival rate for this cancer. However, the length of life of the patients is often not accompanied by the better quality of life due to chemotherapy side effects towards patients’ physical and psychosocial conditions. This study aimed to identify factors that are related to the quality of life of children with acute lymphocytic leukemia who undergo chemotherapy in Dr. M.Djamil Hospital, Padang. Quantitative research with cross sectional approach was used to study 25 children who were recruited using consecutive sampling. The data collection was conducted using PedsQLTM 4.0 Generic Core Scale and nurses roles (Cronbach α = 0,90). The data analysis uses Pearson correlation and independent t-test to examine the relationships between variable and multiple linear regression to identify the factor that is most related with the generic quality of life. The results showed that there was correlation between chemotherapy phases and nurses roles with generic quality of life (p<0.05). Nurses roles is a predictor factors for generic quality of life. Thus, there is a need to improve nurses’ roles through education and training regarding chemotherapy management and its side effects.