TY - GEN
T1 - Extraction of vitexin from binahong (Anredera cordifolia (Ten.) Steenis) leaves using betaine - 1,4 butanediol natural deep eutectic solvent (NADES)
AU - Mulia, Kamarza
AU - Muhammad, Fajri
AU - Krisanti, Elsa
N1 - Publisher Copyright:
© 2017 Author(s).
PY - 2017/3/17
Y1 - 2017/3/17
N2 - The leaves of binahong (Anredera cordifolia (Ten) Steenis) contain flavonoids as bioactive substances that have efficacy to treat wounds and diseases caused by bacteria. One of the flavonoids contained in the leaves is 8-glucopyranosyl-4'5'7-trihydroxyflavone or vitexin. Conventional extraction of flavonoids from leaves of binahong has been developed and usually using non-friendly organic solvent. To overcome these problems, a Natural Deep Eutectic Solvent (NADES) is used to replace the conventional organic solvents, as it is an environmentally friendly, non-toxic and high boiling point solvent. In this study, a betaine-based NADES combined with 1,4-butanediol in 1:3 mole ratio was used as the extraction solvent. Vitexin in the extract was analyzed qualitatively and quantitatively using an HPLC. The extraction of vitexin from binahong leaves at room temperature (27°C) for four hours give yield of 46 ppm, much lower than 200 ppm yield obtained after extraction at 55°C for 90 minutes. This results showed that (a) NADES consisting of betaine and 1,4 butanediol is a promising green solvent for extraction of vitexin from binahong leaves, and, (b) the extraction can be performed above ambient temperature, as long as it does not exceed the degradation temperature of the bioactive compound extracted.
AB - The leaves of binahong (Anredera cordifolia (Ten) Steenis) contain flavonoids as bioactive substances that have efficacy to treat wounds and diseases caused by bacteria. One of the flavonoids contained in the leaves is 8-glucopyranosyl-4'5'7-trihydroxyflavone or vitexin. Conventional extraction of flavonoids from leaves of binahong has been developed and usually using non-friendly organic solvent. To overcome these problems, a Natural Deep Eutectic Solvent (NADES) is used to replace the conventional organic solvents, as it is an environmentally friendly, non-toxic and high boiling point solvent. In this study, a betaine-based NADES combined with 1,4-butanediol in 1:3 mole ratio was used as the extraction solvent. Vitexin in the extract was analyzed qualitatively and quantitatively using an HPLC. The extraction of vitexin from binahong leaves at room temperature (27°C) for four hours give yield of 46 ppm, much lower than 200 ppm yield obtained after extraction at 55°C for 90 minutes. This results showed that (a) NADES consisting of betaine and 1,4 butanediol is a promising green solvent for extraction of vitexin from binahong leaves, and, (b) the extraction can be performed above ambient temperature, as long as it does not exceed the degradation temperature of the bioactive compound extracted.
UR - http://www.scopus.com/inward/record.url?scp=85017516762&partnerID=8YFLogxK
U2 - 10.1063/1.4978091
DO - 10.1063/1.4978091
M3 - Conference contribution
AN - SCOPUS:85017516762
T3 - AIP Conference Proceedings
BT - International Conference on Chemistry, Chemical Process and Engineering, IC3PE 2017
A2 - Fatimah, Is
A2 - Purwiandono, Gani
PB - American Institute of Physics Inc.
T2 - International Conference on Chemistry, Chemical Process and Engineering, IC3PE 2017
Y2 - 15 November 2016 through 16 November 2016
ER -