Extraction of PLA2 and antibacterial activity test of lionfish (Pterois volitans) spine venom

A. N. Sommeng, M. Y.A. Ramadhan, R. Larasati, M. J. Ginting, M. Sahlan, H. Hermansyah, A. Wijanarko

Research output: Contribution to journalConference articlepeer-review

Abstract

The population of Pterois volitans has caused significant damage to other fish populations and coral reef ecosystems. Population control of P. volitans consumes a considerable cost so that the utilization of these fish needs to be sought to be useful along with controlling the population. This fish is known to contain the enzyme Phospholipase A2 (PLA2), which used as an antibiotic against some bacteria. This study will examine the antibacterial activity of the phospholipase A2 enzyme extracted from P. volitans venom to Escherichia coli, Bacillus subtilis, and Staphylococcus aureus. The method used to isolate the enzyme PLA2 is by using precipitation of ammonium sulfate (AS) and precipitation with ethanol. The results of the precipitation tested with the Lowry protein concentration test, the Marinetti PLA2 activity test, and the identification of the SDS-PAGE protein. The agar diffusion disc method is used to test the antibacterial activity. The results obtained from this research are that 80% ammonium sulfate precipitation method has the highest protein and enzyme activity with a ratio of 1.32 times compared to toxic extract. For antibacterial activity test results, an 80% ammonium sulfate sample may inhibit the activity of S. aureus bacteria but does not affect B. subtilis and E. coli.

Original languageEnglish
Article number012040
JournalIOP Conference Series: Earth and Environmental Science
Volume462
Issue number1
DOIs
Publication statusPublished - 15 Apr 2020
Event3rd International Conference on Natural Products and Bioresource Sciences 2019, ICONPROBIOS 2019 - BSD City, Tangerang, Indonesia
Duration: 23 Oct 201924 Oct 2019

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