Extraction of Collagen and Hydroxyapatite from Fish for Bone Scaffold: A Review

Adella Josephin, Nurul Intan, Basari Basari, Siti Fauziyah Rahman

Research output: Contribution to journalConference articlepeer-review


Bones constitute a crucial element of human anatomy. The growing prevalence of bone disorders and diseases, such as fractures caused by osteoporosis, is a matter of concern. Bone tissue engineering, a promising remedy to this problem, involves developing structures using a combination of scaffolds, cells, and biological factors to regenerate bone. The scaffolding materials frequently utilized for bone tissue engineering are collagen and hydroxyapatite, which can be derived from natural sources. Indonesia, the largest archipelagic country, and the world's second-largest fish producer, possesses abundant marine resources. This study discusses the extraction of collagen and hydroxyapatite from fish, which can be used to develop bone scaffold material for treating bone disorders and diseases. Fish-derived collagen extraction can be achieved through acid or pepsin solubilization extraction with or without ultrasonication and salt solubilization extraction. Hydroxyapatite can be extracted using calcination, alkaline heat treatment, enzymatic hydrolysis, and ionic liquid pretreatment. The extraction of collagen and hydroxyapatite is expected to utilize marine and freshwater fish and provide a solution to treat bone disorders and diseases.

Original languageEnglish
Article number090006
JournalAIP Conference Proceedings
Issue number1
Publication statusPublished - 7 Mar 2024
Event15th Asian Congress on Biotechnology in conjunction with the 7th International Symposium on Biomedical Engineering, ACB-ISBE 2022 - Bali, Indonesia
Duration: 2 Oct 20226 Oct 2022


  • bone scaffold
  • collagen
  • fish
  • hydroxyapatite


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