Extraction, antioxidant, and bioactive component assay of lionfish venom Pterois volitans

Andy Noorsaman Sommeng; Rizki Larasati; Mikael Januardi Ginting; Sonya Pebriani; Muhamad Sahlan; Heri Hermansyah; Anondho Wijanarko

doi:10.1063/1.5139345

Extraction, antioxidant, and bioactive component assay of lionfish venom Pterois volitans

Andy Noorsaman Sommeng, Rizki Larasati, Mikael Januardi Ginting, Sonya Pebriani, Muhamad Sahlan, Heri Hermansyah, Anondho Wijanarko

Department of Chemical Engineering

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

1 Citation (Scopus)

Abstract

Lionfish Pterois volitans is a tropical Indo-Pacific native species and invaded on marine ecosystem due to its rapid growth and predatory properties that were highly affecting fish and coral populations. Recent studies show the benefits of natural antioxidants, especially as the prevention of cancer and cardiovascular disease. Crude Venom (CV) was extracted using sonication and precipitated using ethanol and Ammonium Sulfate (AS) with 20, 40, 60, and 80% saturation. The protein concentration of each sample detected using Lowry's method, and SDS-PAGE was done to find out the Molecular Weight (MW) of protein samples. Toxicity analysis to determine the Lethal Concentration (LC₅₀) value was done using Brine Shrimp Lethality Test (BSLT) and antioxidant activity tested using 2,2-diphenyl-1-picrylhydrazyl (DPPH) method to determined Inhibitory Concentration (IC₅₀) value. AS 80% had the higher value of protein concentration 361.76 μg/mL. SDS-PAGE analysis showed that AS 80% had a protein band at 7.9, 46.2 and 52.7 kD which supposed to be the protein that had antioxidant activity. The test showed the LC₅₀ of AS 80% 101.93 μg/mL. Antioxidant analysis showed AS 80% had the higher value of inhibition 57.08 % with IC₅₀ 1.56 mg/mL. This research showed that AS 80% has the highest potential as an antioxidant compound due to its ability to inhibit free radicals.

Original language	English
Title of host publication	4th Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, Health, and Medical Devices
Subtitle of host publication	Proceedings of the International Symposium of Biomedical Engineering, ISBE 2019
Editors	Kenny Lischer, Tomy Abuzairi, Siti Fauziyah Rahman, Misri Gozan
Publisher	American Institute of Physics Inc.
ISBN (Electronic)	9780735419445
DOIs	https://doi.org/10.1063/1.5139345
Publication status	Published - 10 Dec 2019
Event	4th International Symposium of Biomedical Engineering�s Recent Progress in Biomaterials, Drugs Development, Health, and Medical Devices, ISBE 2019 - Padang, West Sumatera, Indonesia Duration: 22 Jul 2019 → 24 Jul 2019

Publication series

Name	AIP Conference Proceedings
Volume	2193
ISSN (Print)	0094-243X
ISSN (Electronic)	1551-7616

Conference

Conference	4th International Symposium of Biomedical Engineering�s Recent Progress in Biomaterials, Drugs Development, Health, and Medical Devices, ISBE 2019
Country/Territory	Indonesia
City	Padang, West Sumatera
Period	22/07/19 → 24/07/19

Keywords

Antioxidant activity
Lionfish
Toxicity analysis

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1063/1.5139345

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Sommeng, A. N., Larasati, R., Ginting, M. J., Pebriani, S., Sahlan, M., Hermansyah, H., & Wijanarko, A. (2019). Extraction, antioxidant, and bioactive component assay of lionfish venom Pterois volitans. In K. Lischer, T. Abuzairi, S. F. Rahman, & M. Gozan (Eds.), 4th Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, Health, and Medical Devices: Proceedings of the International Symposium of Biomedical Engineering, ISBE 2019 Article 030008 (AIP Conference Proceedings; Vol. 2193). American Institute of Physics Inc.. https://doi.org/10.1063/1.5139345

Sommeng, Andy Noorsaman ; Larasati, Rizki ; Ginting, Mikael Januardi et al. / Extraction, antioxidant, and bioactive component assay of lionfish venom Pterois volitans. 4th Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, Health, and Medical Devices: Proceedings of the International Symposium of Biomedical Engineering, ISBE 2019. editor / Kenny Lischer ; Tomy Abuzairi ; Siti Fauziyah Rahman ; Misri Gozan. American Institute of Physics Inc., 2019. (AIP Conference Proceedings).

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title = "Extraction, antioxidant, and bioactive component assay of lionfish venom Pterois volitans",

abstract = "Lionfish Pterois volitans is a tropical Indo-Pacific native species and invaded on marine ecosystem due to its rapid growth and predatory properties that were highly affecting fish and coral populations. Recent studies show the benefits of natural antioxidants, especially as the prevention of cancer and cardiovascular disease. Crude Venom (CV) was extracted using sonication and precipitated using ethanol and Ammonium Sulfate (AS) with 20, 40, 60, and 80% saturation. The protein concentration of each sample detected using Lowry's method, and SDS-PAGE was done to find out the Molecular Weight (MW) of protein samples. Toxicity analysis to determine the Lethal Concentration (LC50) value was done using Brine Shrimp Lethality Test (BSLT) and antioxidant activity tested using 2,2-diphenyl-1-picrylhydrazyl (DPPH) method to determined Inhibitory Concentration (IC50) value. AS 80% had the higher value of protein concentration 361.76 μg/mL. SDS-PAGE analysis showed that AS 80% had a protein band at 7.9, 46.2 and 52.7 kD which supposed to be the protein that had antioxidant activity. The test showed the LC50 of AS 80% 101.93 μg/mL. Antioxidant analysis showed AS 80% had the higher value of inhibition 57.08 % with IC50 1.56 mg/mL. This research showed that AS 80% has the highest potential as an antioxidant compound due to its ability to inhibit free radicals.",

keywords = "Antioxidant activity, Lionfish, Toxicity analysis",

author = "Sommeng, {Andy Noorsaman} and Rizki Larasati and Ginting, {Mikael Januardi} and Sonya Pebriani and Muhamad Sahlan and Heri Hermansyah and Anondho Wijanarko",

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year = "2019",

month = dec,

day = "10",

doi = "10.1063/1.5139345",

language = "English",

series = "AIP Conference Proceedings",

publisher = "American Institute of Physics Inc.",

editor = "Kenny Lischer and Tomy Abuzairi and Rahman, {Siti Fauziyah} and Misri Gozan",

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Sommeng, AN, Larasati, R, Ginting, MJ, Pebriani, S, Sahlan, M , Hermansyah, H & Wijanarko, A 2019, Extraction, antioxidant, and bioactive component assay of lionfish venom Pterois volitans. in K Lischer, T Abuzairi, SF Rahman & M Gozan (eds), 4th Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, Health, and Medical Devices: Proceedings of the International Symposium of Biomedical Engineering, ISBE 2019., 030008, AIP Conference Proceedings, vol. 2193, American Institute of Physics Inc., 4th International Symposium of Biomedical Engineering�s Recent Progress in Biomaterials, Drugs Development, Health, and Medical Devices, ISBE 2019, Padang, West Sumatera, Indonesia, 22/07/19. https://doi.org/10.1063/1.5139345

Extraction, antioxidant, and bioactive component assay of lionfish venom Pterois volitans. / Sommeng, Andy Noorsaman; Larasati, Rizki; Ginting, Mikael Januardi et al.
4th Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, Health, and Medical Devices: Proceedings of the International Symposium of Biomedical Engineering, ISBE 2019. ed. / Kenny Lischer; Tomy Abuzairi; Siti Fauziyah Rahman; Misri Gozan. American Institute of Physics Inc., 2019. 030008 (AIP Conference Proceedings; Vol. 2193).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - Extraction, antioxidant, and bioactive component assay of lionfish venom Pterois volitans

AU - Sommeng, Andy Noorsaman

AU - Larasati, Rizki

AU - Ginting, Mikael Januardi

AU - Pebriani, Sonya

AU - Sahlan, Muhamad

AU - Hermansyah, Heri

AU - Wijanarko, Anondho

PY - 2019/12/10

Y1 - 2019/12/10

N2 - Lionfish Pterois volitans is a tropical Indo-Pacific native species and invaded on marine ecosystem due to its rapid growth and predatory properties that were highly affecting fish and coral populations. Recent studies show the benefits of natural antioxidants, especially as the prevention of cancer and cardiovascular disease. Crude Venom (CV) was extracted using sonication and precipitated using ethanol and Ammonium Sulfate (AS) with 20, 40, 60, and 80% saturation. The protein concentration of each sample detected using Lowry's method, and SDS-PAGE was done to find out the Molecular Weight (MW) of protein samples. Toxicity analysis to determine the Lethal Concentration (LC50) value was done using Brine Shrimp Lethality Test (BSLT) and antioxidant activity tested using 2,2-diphenyl-1-picrylhydrazyl (DPPH) method to determined Inhibitory Concentration (IC50) value. AS 80% had the higher value of protein concentration 361.76 μg/mL. SDS-PAGE analysis showed that AS 80% had a protein band at 7.9, 46.2 and 52.7 kD which supposed to be the protein that had antioxidant activity. The test showed the LC50 of AS 80% 101.93 μg/mL. Antioxidant analysis showed AS 80% had the higher value of inhibition 57.08 % with IC50 1.56 mg/mL. This research showed that AS 80% has the highest potential as an antioxidant compound due to its ability to inhibit free radicals.

AB - Lionfish Pterois volitans is a tropical Indo-Pacific native species and invaded on marine ecosystem due to its rapid growth and predatory properties that were highly affecting fish and coral populations. Recent studies show the benefits of natural antioxidants, especially as the prevention of cancer and cardiovascular disease. Crude Venom (CV) was extracted using sonication and precipitated using ethanol and Ammonium Sulfate (AS) with 20, 40, 60, and 80% saturation. The protein concentration of each sample detected using Lowry's method, and SDS-PAGE was done to find out the Molecular Weight (MW) of protein samples. Toxicity analysis to determine the Lethal Concentration (LC50) value was done using Brine Shrimp Lethality Test (BSLT) and antioxidant activity tested using 2,2-diphenyl-1-picrylhydrazyl (DPPH) method to determined Inhibitory Concentration (IC50) value. AS 80% had the higher value of protein concentration 361.76 μg/mL. SDS-PAGE analysis showed that AS 80% had a protein band at 7.9, 46.2 and 52.7 kD which supposed to be the protein that had antioxidant activity. The test showed the LC50 of AS 80% 101.93 μg/mL. Antioxidant analysis showed AS 80% had the higher value of inhibition 57.08 % with IC50 1.56 mg/mL. This research showed that AS 80% has the highest potential as an antioxidant compound due to its ability to inhibit free radicals.

KW - Antioxidant activity

KW - Lionfish

KW - Toxicity analysis

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DO - 10.1063/1.5139345

M3 - Conference contribution

AN - SCOPUS:85076751785

T3 - AIP Conference Proceedings

BT - 4th Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, Health, and Medical Devices

A2 - Lischer, Kenny

A2 - Abuzairi, Tomy

A2 - Rahman, Siti Fauziyah

A2 - Gozan, Misri

PB - American Institute of Physics Inc.

T2 - 4th International Symposium of Biomedical Engineering�s Recent Progress in Biomaterials, Drugs Development, Health, and Medical Devices, ISBE 2019

Y2 - 22 July 2019 through 24 July 2019

ER -

Sommeng AN, Larasati R, Ginting MJ, Pebriani S, Sahlan M , Hermansyah H et al. Extraction, antioxidant, and bioactive component assay of lionfish venom Pterois volitans. In Lischer K, Abuzairi T, Rahman SF, Gozan M, editors, 4th Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, Health, and Medical Devices: Proceedings of the International Symposium of Biomedical Engineering, ISBE 2019. American Institute of Physics Inc. 2019. 030008. (AIP Conference Proceedings). doi: 10.1063/1.5139345

Extraction, antioxidant, and bioactive component assay of lionfish venom Pterois volitans

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Keywords

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