Extraction, antioxidant, and bioactive component assay of lionfish venom Pterois volitans

Andy Noorsaman Sommeng, Rizki Larasati, Mikael Januardi Ginting, Sonya Pebriani, Muhamad Sahlan, Heri Hermansyah, Anondho Wijanarko

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

1 Citation (Scopus)

Abstract

Lionfish Pterois volitans is a tropical Indo-Pacific native species and invaded on marine ecosystem due to its rapid growth and predatory properties that were highly affecting fish and coral populations. Recent studies show the benefits of natural antioxidants, especially as the prevention of cancer and cardiovascular disease. Crude Venom (CV) was extracted using sonication and precipitated using ethanol and Ammonium Sulfate (AS) with 20, 40, 60, and 80% saturation. The protein concentration of each sample detected using Lowry's method, and SDS-PAGE was done to find out the Molecular Weight (MW) of protein samples. Toxicity analysis to determine the Lethal Concentration (LC50) value was done using Brine Shrimp Lethality Test (BSLT) and antioxidant activity tested using 2,2-diphenyl-1-picrylhydrazyl (DPPH) method to determined Inhibitory Concentration (IC50) value. AS 80% had the higher value of protein concentration 361.76 μg/mL. SDS-PAGE analysis showed that AS 80% had a protein band at 7.9, 46.2 and 52.7 kD which supposed to be the protein that had antioxidant activity. The test showed the LC50 of AS 80% 101.93 μg/mL. Antioxidant analysis showed AS 80% had the higher value of inhibition 57.08 % with IC50 1.56 mg/mL. This research showed that AS 80% has the highest potential as an antioxidant compound due to its ability to inhibit free radicals.

Original languageEnglish
Title of host publication4th Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, Health, and Medical Devices
Subtitle of host publicationProceedings of the International Symposium of Biomedical Engineering, ISBE 2019
EditorsKenny Lischer, Tomy Abuzairi, Siti Fauziyah Rahman, Misri Gozan
PublisherAmerican Institute of Physics Inc.
ISBN (Electronic)9780735419445
DOIs
Publication statusPublished - 10 Dec 2019
Event4th International Symposium of Biomedical Engineering�s Recent Progress in Biomaterials, Drugs Development, Health, and Medical Devices, ISBE 2019 - Padang, West Sumatera, Indonesia
Duration: 22 Jul 201924 Jul 2019

Publication series

NameAIP Conference Proceedings
Volume2193
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616

Conference

Conference4th International Symposium of Biomedical Engineering�s Recent Progress in Biomaterials, Drugs Development, Health, and Medical Devices, ISBE 2019
CountryIndonesia
CityPadang, West Sumatera
Period22/07/1924/07/19

Keywords

  • Antioxidant activity
  • Lionfish
  • Toxicity analysis

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